Anti-Maxi Potassium channel beta 抗体 (ab3587)

製品の概要

  • 製品名
    Anti-Maxi Potassium channel beta antibody
    Maxi Potassium channel beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Maxi Potassium channel beta
  • 特異性
    ab3587 is specific for the Beta-1 subunit of Maxi K+. It does not detect the beta-2 subunit
  • アプリケーション
    適用あり: IHC-P, WB, ICC, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Horse, Chicken, Dog, Human, Pig
    交差が予測される動物種: Cow
  • 免疫原

    Synthetic peptide corresponding to Human Maxi Potassium channel beta aa 90-103.
    Sequence:

    LYHTEDTRDQNQQC


    (Peptide available as ab5023)

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 一次抗体 備考
    Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open. Maxi K+ beta is the target for possible therapeutics because of its role in blood flow and blood pressure regulation.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200.
WB Use a concentration of 2 µg/ml. Can be blocked with Maxi Potassium channel beta peptide (ab5023). Detects an ~28 kDa protein representing Maxi K+ beta from mouse myometrial smooth muscle cell extracts.
ICC Use at an assay dependent concentration.
ICC/IF 1/100. PubMed: 16116057

ターゲット情報

  • 関連性
    MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits.
  • 細胞内局在
    Membrane; multi-pass membrane protein
  • 参照データベース
  • 別名
    • BK channel beta subunit antibody
    • BK channel subunit beta 1 antibody
    • BKbeta antibody
    • BKbeta1 antibody
    • Calcium activated potassium channel subfamily M subunit beta 1 antibody
    • Calcium activated potassium channel subunit beta 1 antibody
    • Calcium activated potassium channel subunit beta antibody
    • Charybdotoxin receptor subunit beta 1 antibody
    • Hbeta1 antibody
    • hslo beta antibody
    • K(VCA)beta 1 antibody
    • K(VCA)beta antibody
    • KCNMB 1 antibody
    • KCNMB1 antibody
    • Large conductance Ca2+ activated K+ channel beta 1 subunit antibody
    • Maxi K channel beta subunit antibody
    • Maxi K channel subunit beta 1 antibody
    • Potassium large conductance calcium activated channel subfamily M beta member 1 antibody
    • Slo beta 1 antibody
    • SLO beta antibody
    see all

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参考文献

This product has been referenced in:
  • Nystoriak MA  et al. Ser1928 phosphorylation by PKA stimulates the L-type Ca2+ channel CaV1.2 and vasoconstriction during acute hyperglycemia and diabetes. Sci Signal 10:N/A (2017). Read more (PubMed: 28119464) »
  • Wang Y  et al. The Involvement of Ca(2+) Signal Pathways in Distal Colonic Myocytes in a Rat Model of Dextran Sulfate Sodium-induced Colitis. Chin Med J (Engl) 129:1185-92 (2016). WB . Read more (PubMed: 27174327) »

See all 13 Publications for this product

レビューと Q&A

Thank you for contacting us.
We do have the blocking peptide available as ab5023.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (Cartilage, articular)
Specification
Cartilage, articular
Fixative
Neutral buffered formalin
Antigen retrieval step
None
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Ali Mobasheri

Verified customer

投稿 Feb 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Horse Cell lysate - whole cell (Chondrocytes isolated from equine cartilage)
Loading amount
25 µg
Specification
Chondrocytes isolated from equine cartilage
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Ali Mobasheri

Verified customer

投稿 Jan 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Chicken Tissue lysate - other (cerebellum)
Loading amount
22 µg
Specification
cerebellum
Blocking step
Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100%
Username

Dr. Soledad Miranda-Rottmann

Verified customer

投稿 Jul 25 2006

Thank you for your enquiry. Further to correspondence with the source of this antibody I have determined that ab3587 is specific for the Beta-1 subunit of Maxi K+. Further to a sequence comparison between the immunogen and the sequence of the beta-...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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