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Fusion protein, corresponding to C terminal amino acids 690-1196 of Mouse Maxi Potassium channel alpha (AAA39746).
Our Abpromise guarantee covers the use of ab99046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.1 - 10 µg/ml. 0.1-1.0ug/mL (Perox), 1.0-10ug/mL (IF)|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 110-130 kDa (predicted molecular weight: 134 kDa). 1 µg/mL of ab99046 was sufficient for detection of Maxi Potassium channel alpha in 20µg of mouse brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 0.1 - 1 µg/ml.|
|IHC-Fr||Use a concentration of 0.1 - 1 µg/ml.|
|Flow Cyt||Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
ab99046 staining Maxi Potassium channel alpha in Mouse cerebellum tissue sections by Immunohistochemistry.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"