製品の概要

  • 製品名Anti-MAX antibody [73C5a] - ChIP Grade
    MAX 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [73C5a] to MAX - ChIP Grade
  • アプリケーション適用あり: Flow Cyt, ChIP, WB, Dot blotmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant human MAX

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ChIP Use at an assay dependent concentration. PubMed: 20797623
WB Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 18 kDa).
Dot blot Use at an assay dependent concentration.

ターゲット情報

  • 関連性MAX is a basic helix-loop-helix, leucine zipper protein that forms heterodimers with MYC and MAD. It is involved in regulating gene expression.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • bHLHd4 antibody
    • bHLHd5 antibody
    • bHLHd6 antibody
    • bHLHd7 antibody
    • bHLHd8 antibody
    • Helix loop helix zipper protein antibody
    • MAX protein antibody
    • MGC10775 antibody
    • MGC11225 antibody
    • MGC18164 antibody
    • MGC34679 antibody
    • MGC36767 antibody
    • MYC associated factor X antibody
    • Myc binding novel HLH/LZ protein antibody
    • Orf 1 antibody
    • Orf1 antibody
    • Protein max antibody
    see all

Anti-MAX antibody [73C5a] - ChIP Grade 画像

  • Anti-MAX antibody [73C5a] - ChIP Grade (ab53570) + immunised recombinant protein

    Predicted band size : 18 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing A549 cells stained with ab53570 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53570, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-MAX antibody [73C5a] - ChIP Grade (ab53570) 使用論文

This product has been referenced in:
  • Wang M  et al. Common genetic variation in ETV6 is associated with colorectal cancer susceptibility. Nat Commun 7:11478 (2016). ChIP ; Human . Read more (PubMed: 27145994) »
  • Xue G  et al. c-Myc-mediated repression of miR-15-16 in hypoxia is induced by increased HIF-2a and promotes tumor angiogenesis and metastasis by upregulating FGF2. Oncogene N/A:N/A (2014). Read more (PubMed: 24704828) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (skin keratinocytes)
Gel Running Conditions Reduced Denaturing (AnyKd from BioRad)
Loading amount 40 µg
Specification skin keratinocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Julien Coutier

Verified customer

投稿 Feb 07 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"