Anti-MAVS 抗体 (ab89825)
Key features and details
- Mouse polyclonal to MAVS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MAVS antibody
MAVS 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to MAVS -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human MAVS. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- Human placenta tissue lysate. Cell lysate from transfected 293T cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab89825の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa. |
ターゲット情報
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機能
Required for innate immune defense against viruses. Acts downstream of DDX58 and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis. -
組織特異性
Present in T-cells, monocytes, epithelial cells and hepatocytes (at protein level). Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes. -
配列類似性
Contains 1 CARD domain. -
ドメイン
Both CARD and transmembrane domains are essential for antiviral function. The CARD domain is responsible for interaction with DDX58 and IFIH1. -
翻訳後修飾
Ubiquitinated; undergoes 'Lys-48'-linked polyubiquitination catalyzed by ITCH; ITCH-dependent polyubiquitination is mediated by the interaction with PCBP2 and leads to MAVS proteasomal degradation. -
細胞内局在
Mitochondrion outer membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 57506 Human
- Omim: 609676 Human
- SwissProt: Q7Z434 Human
- Unigene: 570362 Human
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別名
- CARD adapter inducing interferon beta antibody
- CARD adaptor inducing IFN beta antibody
- Cardif antibody
see all
画像
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Anti-MAVS antibody (ab89825) at 1 µg/ml + Human placenta tissue lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: ~57 kDa why is the actual band size different from the predicted? -
All lanes : Anti-MAVS antibody (ab89825) at 1 µg/ml
Lane 1 : cell lysate from MAVS transfected 293T cells
Lane 2 : cell lysate from non transfected 293T cells
Lysates/proteins at 25 µg per lane.
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: ~75 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (1)
ab89825 は 1 報の論文で使用されています。
- Chang AY et al. Modulation of SF3B1 in the pre-mRNA spliceosome induces a RIG-I-dependent type I IFN response. J Biol Chem 297:101277 (2021). PubMed: 34619148