製品の概要

  • 製品名
    Anti-Matriptase 2 antibody
    Matriptase 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Matriptase 2
  • 特異性
    ab28287 recognises the stem region of Matriptase 2. It does not react with the free enzyme. It does not recognise Matriptase 1.
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to the stem region of human Matriptase 2.

    (Peptide available as ab41267.)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Predicted molecular weight: 89 kDa. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能
    Serine protease which hydrolyzes a range of proteins including type I collagen, fibronectin and fibrinogen. Can also activate urokinase-type plasminogen activator with low efficiency. May play a specialized role in matrix remodeling processes in liver. Required to sense iron deficiency. Overexpression suppresses activation of the HAMP promoter.
  • 組織特異性
    Liver specific.
  • 関連疾患
    Defects in TMPRSS6 are the cause of iron-refractory iron deficiency anemia (IRIDA) [MIM:206200]; also known as hypochromic microcytic anemia with defect in iron metabolism or hereditary iron-handling disorder or pseudo-iron-deficiency anemia. Key features include congenital hypochromic microcytic anemia, very low mean corpuscular erythrocyte volume, low transferrin saturation, abnormal iron absorption characterized by no hematologic improvement following treatment with oral iron, and abnormal iron utilization characterized by a sluggish, incomplete response to parenteral iron.
  • 配列類似性
    Belongs to the peptidase S1 family.
    Contains 2 CUB domains.
    Contains 3 LDL-receptor class A domains.
    Contains 1 peptidase S1 domain.
  • ドメイン
    Cytoplasmic domain mediates HAMP suppression via proximal promoter element(s).
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • IRIDA antibody
    • Matriptase-2 antibody
    • Membrane bound mosaic serine proteinase matriptase 2 antibody
    • Membrane type serine proteinase 2 antibody
    • MTSP 2 antibody
    • MTSP2 antibody
    • PVAE354 antibody
    • TMPRSS 6 antibody
    • TMPRSS6 antibody
    • TMPS6_HUMAN antibody
    • TMSP 6 antibody
    • TMSP6 antibody
    • Transmembrane protease serine 6 antibody
    • Type II Membrane Serine Proteinase 6 antibody
    see all

画像

  • ICC/IF image of ab28287 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28287, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

プロトコール

参考文献

This product has been referenced in:
  • Gnana-Prakasam JP  et al. Retinal expression of the serine protease matriptase-2 (Tmprss6) and its role in retinal iron homeostasis. Mol Vis 20:561-74 (2014). Mouse . Read more (PubMed: 24791141) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (LNCaP - prostate cancer cells - plasma membrane fr)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
20 µg
Specification
LNCaP - prostate cancer cells - plasma membrane fr
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

投稿 Sep 26 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (PC-3 prostate cancer cells)
Permeabilization
No
Specification
PC-3 prostate cancer cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Methanol
Username

Dr. Armen Petrosyan

Verified customer

投稿 Feb 17 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat hepatocytes)
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Loading amount
20 µg
Specification
Rat hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

投稿 Jan 11 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

投稿 Dec 30 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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