製品の概要

  • 製品名Anti-Matrin 3 antibody
    Matrin 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Matrin 3
  • アプリケーション適用あり: IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Chinese Hamster, Orangutan, Bat
  • 免疫原

    Synthetic peptide designed within residues: GFYCKLCSLF YTNEEVAKNT HCSSLPHYQK LKKFLNKLAE ERRQKKET, corresponding to C terminal amino acids 800-847 of Human Matrin 3 (NP_061322.2)

  • ポジティブ・コントロール
    • Human: breast carcinoma, colon carcinoma, laryngeal squamous cell carcinoma, linitis plastica stomach cancer, lung adenocarcinoma, metastatic lymph node, non-small cell lung cancer, ovarian carcinoma, pancreatic islet cell tumor, prostate carcinoma, skin basal cell carcinoma, skin squamous cell carcinoma, small cell lung cancer, stomach adenocarcinoma, testicular seminoma. Mouse: squamous cell carcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Note: expected to work on frozen sections.
ICC/IF 1/100.

ターゲット情報

  • 機能May play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. In association with the SFPQ-NONO heteromer may play a role in nuclear retention of defective RNAs.
  • 関連疾患Defects in MATR3 are the cause of myopathy distal type 2 (MPD2) [MIM:606070]; also called vocal cord and pharyngeal dysfunction with distal myopathy (VCPDM). MPD2 is a muscular disorder characterized by distal weakness, with onset in hands and feet, associated with vocal cord and pharyngeal weakness causing a nasal voice and swallowing disorders.
  • 配列類似性Contains 1 matrin-type zinc finger.
    Contains 2 RRM (RNA recognition motif) domains.
  • 細胞内局在Nucleus matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALS21 antibody
    • KIAA0723 antibody
    • Matr3 antibody
    • MATR3_HUMAN antibody
    • Matrin-3 antibody
    • Matrin3 antibody
    • MPD2 antibody
    • VCPDM antibody
    • Vocal cord and pharyngeal weakness with distal myopathy antibody
    see all

Anti-Matrin 3 antibody 画像

  • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human colon adenocarcinoma using 1/250 ab84422, followed by DAB staining.
  • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Mouse squamous cell carcinoma using 1/250 ab84422, followed by DAB staining.
  • Detection of Human Matrin 3 by Immunofluorescence.
    Sample: FFPE section of human breast carcinoma.
    Antibody: ab84422 used at a dilution of 1/100.
    Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1/500.
  • Detection of Human Matrin 3 by Immunofluorescence.
    Sample: FFPE section of Human breast carcinoma.
    Antibody: ab84422 used at a dilution of 1/100.
    Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1/500.
  • ab84422 staining Matrin 3 in human U2OS cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0.5% NP-40, blocked with 1% BSA for 1 hour a room temperature, then incubated with ab84422 at a 1/100 dilution for 1 hour. The secondary antibody used was an Alexa-Fluor 488 conjugate donkey polyclonal, used at a 1/1000 dilution.

    See Abreview

Anti-Matrin 3 antibody (ab84422) 使用論文

ab84422 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS)
Specification U2OS
Fixative Formaldehyde
Permeabilization Yes - 0.5% NP40/PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 27 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS cells)
Specification U2OS cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% NP-40
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Leilani Beltran

Verified customer

投稿 Sep 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"