Anti-Matrin 3 抗体 (ab70336)
Key features and details
- Rabbit polyclonal to Matrin 3
- Suitable for: IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Matrin 3 antibody
Matrin 3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Matrin 3 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
免疫原
Synthetic peptide corresponding to a region between residues 475 and 500 of human Matrin 3 (NP_061322.2)
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ポジティブ・コントロール
- HeLa whole cell lysate (ab150035).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.764% Sodium citrate, 1.815% Tris, 0.021% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab70336 was affinity purified using an epitope specific to Matrin 3 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70336の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP | (1) |
Use at 1-4 µg/mg of lysate.
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特記事項 |
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at 1-4 µg/mg of lysate. |
ターゲット情報
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機能
May play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. In association with the SFPQ-NONO heteromer may play a role in nuclear retention of defective RNAs. -
関連疾患
Defects in MATR3 are the cause of myopathy distal type 2 (MPD2) [MIM:606070]; also called vocal cord and pharyngeal dysfunction with distal myopathy (VCPDM). MPD2 is a muscular disorder characterized by distal weakness, with onset in hands and feet, associated with vocal cord and pharyngeal weakness causing a nasal voice and swallowing disorders. -
配列類似性
Contains 1 matrin-type zinc finger.
Contains 2 RRM (RNA recognition motif) domains. -
細胞内局在
Nucleus matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 9782 Human
- Entrez Gene: 17184 Mouse
- Entrez Gene: 29150 Rat
- Omim: 164015 Human
- SwissProt: P43243 Human
- SwissProt: Q8K310 Mouse
- SwissProt: P43244 Rat
- Unigene: 268939 Human
see all -
別名
- ALS21 antibody
- KIAA0723 antibody
- Matr3 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryengeal squamous cell carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling Matrin 3 with ab70336 at 1/1000 (1µg/ml). Detection: DAB.
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Detection of Human Matrin 3 by Western Blot of Immunprecipitate. Lane 1: HeLa whole cell lysates immunoprecipitated using ab70336 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 2: an irrelevant antibody was used for immunoprecipitation. All lanes: ab70336 at 3µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab70336 は 4 報の論文で使用されています。
- Boehringer A et al. ALS Associated Mutations in Matrin 3 Alter Protein-Protein Interactions and Impede mRNA Nuclear Export. Sci Rep 7:14529 (2017). PubMed: 29109432
- Li Y et al. Immunoprecipitation and mass spectrometry defines an extensive RBM45 protein-protein interaction network. Brain Res 1647:79-93 (2016). PubMed: 26979993
- Quintero-Rivera F et al. MATR3 disruption in human and mouse associated with bicuspid aortic valve, aortic coarctation and patent ductus arteriosus. Hum Mol Genet 24:2375-89 (2015). WB . PubMed: 25574029
- Kula A et al. Characterization of the HIV-1 RNA associated proteome identifies Matrin 3 as a nuclear cofactor of Rev function. Retrovirology 8:60 (2011). IP ; Human . PubMed: 21771346