製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Activates E box-dependent transcription in collaboration with TCF3/E47. May be a trans-acting factor involved in the development and maintenance of the mammalian nervous system. Transactivates the promoter of its own gene.
  • 配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Atoh 2 antibody
    • Atoh2 antibody
    • Atonal homolog 2 antibody
    • Atonal protein homolog 2 antibody
    • Atonal protein homolog2 antibody
    • bHLH a2 antibody
    • bHLHa 2 antibody
    • bHLHa2 antibody
    • Class A basic helix-loop-helix protein 2 antibody
    • Helix loop helix protein mATH 2 antibody
    • Helix loop helix protein mATH2 antibody
    • MATH 2 antibody
    • Math2 antibody
    • NDF 6 antibody
    • NDF6 antibody
    • NDF6_HUMAN antibody
    • Neuro D6 antibody
    • NeuroD6 antibody
    • Neurogenic differentiation 6 antibody
    • Neurogenic differentiation factor 6 antibody
    • Nex 1 antibody
    • Nex 1m antibody
    • Nex antibody
    • Nex1 antibody
    • Nex1 m antibody
    • Nex1m antibody
    • Protein atonal homolog 2 antibody
    • Protein atonal homolog2 antibody
    see all

Anti-MATH2/NEUROD6 antibody 画像

  • All lanes : Anti-MATH2/NEUROD6 antibody (ab85824) at 1 µg/ml

    Lane 1 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 2 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Brain (Rat) Tissue Lysate
    Lane 5 : Human brain tissue lysate - total protein (ab29466)
    Lane 6 : U373 MG (Human glioblastoma-astrocytoma, epithelial-like cell line) Nuclear Lysate (ab14902)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,120 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes
  • ICC/IF image of ab85824 stained PC12 cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85824 at 5ug/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti- Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MATH2/NEUROD6 antibody (ab85824) 使用論文

This product has been referenced in:
  • Klisch TJ  et al. In vivo Atoh1 targetome reveals how a proneural transcription factor regulates cerebellar development. Proc Natl Acad Sci U S A 108:3288-93 (2011). ICC/IF ; Mouse . Read more (PubMed: 21300888) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"