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Our Abpromise guarantee covers the use of ab27667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC (PFA fixed)||1/1000. Mouse embryos were fixed in 4% PFA in phosphate buffer for 2 h at 4 degrees. Rinsed well with phosphate buffer including an overnight rinse (all at 4 degrees). Sunk in 30% sucrose/phos buffer and embedded in OCT for cryosection. Inconclusive results obtained by WB (please see abreview)|
|ICC/IF||Use at an assay dependent concentration.|
ab27667 staining mouse adrenal tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking. The primary antibody was diluted 1/200 and incubated with the sample for 40 minutes at 20°C. A HRP conjugated goat anti-mouse antibody was used as the secondary.