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Our Abpromise guarantee covers the use of ab85513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling MATH1/HATH1 with ab85513.
ab85513 staining MATH1/HATH1 in murine cochlea tissue by Immunohistochemistry.Tissue was fixed in 4% paraformaldehyde, blocked with 2.5% NGS/ 0.5% Triton X-100 for 1 hour and then incubated with ab85513 at a 1/500 dilution for 24 hours at 4°C. The Alexa-Fluor 633 conjugated secondary was used at a 1/500 dilution. Top image: SG spiral ganglion, OC organ of corti. Middle image: CHCs crista hair cells and the organ of corti. Bottom image: HC hair cells.
ab85513 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"