製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MAT2B
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human MAT2B.

  • ポジティブ・コントロール
    • WB: Human liver and fetal liver tissue lysates and HeLa, Jurkat, HEk293, HepG2 and NIH3T3 whole cell lysates. ICC/IF: formaldehyde fixed HeLa cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab109484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 37 kDa).

ターゲット情報

  • 機能
    Non-catalytic regulatory subunit of S-adenosylmethionine synthetase 2 (MAT2A), an enzyme that catalyzes the formation of S-adenosylmethionine from methionine and ATP. Regulates the activity of S-adenosylmethionine synthetase 2 by changing its kinetic properties, rendering the enzyme more susceptible to S-adenosylmethionine inhibition.
  • 組織特異性
    Widely expressed.
  • パスウェイ
    Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
  • 配列類似性
    Belongs to the dTDP-4-dehydrorhamnose reductase family. MAT2B subfamily.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1110064C04Rik antibody
    • 2410018D16Rik antibody
    • AI182287 antibody
    • AU022853 antibody
    • Beta regulatory subunit of methionine adenosyltransferase antibody
    • dTDP 4 keto 6 deoxy D glucose 4 reductase antibody
    • DTDP-4-keto-6-deoxy-D-glucose 4-reductase antibody
    • MAT II antibody
    • MAT II beta antibody
    • Mat2b antibody
    • MAT2B_HUMAN antibody
    • MATIIbeta antibody
    • Methionine adenosyltransferase 2 beta subunit antibody
    • Methionine adenosyltransferase 2 subunit beta antibody
    • Methionine adenosyltransferase II beta antibody
    • MGC12237 antibody
    • MSTP045 antibody
    • Nbla02999 antibody
    • OTTMUSP00000005600 antibody
    • Putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase antibody
    • Putative protein product of Nbla02999 antibody
    • RP23-382C18.2 antibody
    • SDR23E1 antibody
    • Short chain dehydrogenase/reductase family 23E member 1 antibody
    • TGR antibody
    see all

画像

  • All lanes : Anti-MAT2B antibody (ab109484) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : Human liver tissue lysate - total protein (ab29889)
    Lane 7 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)


    Exposure time : 90 seconds
  • ICC/IF image of ab109484 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109484 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:

See 1 Publication for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab109484.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録