製品の概要

  • 製品名Anti-MAT tag antibody [MAT1-87]
  • 製品の詳細
    Mouse monoclonal [MAT1-87] to MAT tag
  • 特異性ab50292 recognizes the seven amino acid MAT epitope sequence within either N or C terminal MAT-tagged fusion proteins
  • アプリケーション適用あり: ICC/IF, WB, IP, ELISAmore details
  • 免疫原

    A synthetic MAT peptide

    HNHRHKHGGGC

    conjugated to KLH.

  • ポジティブ・コントロール
    • Cell extracts expressing a C-terminal MAT-tagged protein.
  • 特記事項


    Monoclonal antibodies reacting specifically with the MAT tag may be useful in various immuno detection techniques to identify the expression of a MAT fusion protein or to aid in isolation and purification when MAT fusion proteins are produced in prokaryotic or eukaryotic host cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)Purified immunoglobulin
  • 一次抗体 備考Monoclonal antibodies reacting specifically with the MAT tag may be useful in various immuno detection techniques to identify the expression of a MAT fusion protein or to aid in isolation and purification when MAT fusion proteins are produced in prokaryotic or eukaryotic host cells.
  • ポリ/モノモノクローナル
  • クローン名MAT1-87
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab50292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 1 kDa.
IP Use a concentration of 5 - 10 µg/ml.

5 - 10 µg can immunoprecipitate a recombinant C-terminal MAT-tagged protein.

ELISA Use at an assay dependent concentration.

ターゲット情報

  • 関連性The MAT tag is a seven amino acid peptide tag that binds to transition metals such as Nickel and Cobalt. This tag, which consists of the sequence HNHRHKH, allows the purification of MAT fusion proteins using immobilized metal affinity chromatography (IMAC) such as the highly selective HIS-Select® Nickel Affinity Gel. The MAT tag sequence can be incorporated into both prokaryotic and eukaryotic expression vectors.

Anti-MAT tag antibody [MAT1-87] (ab50292) 使用論文

ab50292 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"