製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MASTL
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding a region between amino acids 550-600 of human MASTL (NP_116233.2).

  • ポジティブ・コントロール
    • Hela and 293T whole cell lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 97 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.
  • 関連疾患
    Defects in MASTL are the cause of thrombocytopenia type 2 (THC2) [MIM:188000]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
  • 配列類似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Phosphorylation at Thr-741 by CDK1 during M phase activates its kinase activity (By similarity). Maximum phosphorylation occurs in prometaphase.
  • 細胞内局在
    Cytoplasm > cytoskeleton > centrosome. Nucleus. Cleavage furrow. During interphase is mainly nuclear, upon nuclear envelope breakdown localizes at the cytoplasm and during mitosis at the centrosomes. Upon mitotic exit moves to the cleavage furrow.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2700091H24Rik antibody
    • C88295 antibody
    • FLJ14813 antibody
    • GREATWALL antibody
    • Greatwall protein kinase antibody
    • GW antibody
    • GWL antibody
    • GWL_HUMAN antibody
    • hGWL antibody
    • MAST-L antibody
    • Mastl antibody
    • MGC117975 antibody
    • Microtubule associated serine/threonine kinase like antibody
    • Microtubule-associated serine/threonine-protein kinase-like antibody
    • RP11 85G18.2 antibody
    • Serine/threonine-protein kinase greatwall antibody
    • THC2 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma (left) and lung carcinoma (right) tissues labelling MASTL with ab86387 at 1/1000 (1µg/ml) and 1/500 (2µg/ml). Detection: DAB and a DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100).
  • All lanes : Anti-MASTL antibody (ab86387) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 97 kDa
    Observed band size : 118 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Detection of MASTL by Western blot of Immunprecipitate.
    ab86387, at 1 µg/ml, staining MASTL in HeLa whole cell lysate immunoprecipitated using ab86387 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with an exposure time of 10 seconds.

参考文献

This product has been referenced in:
  • Anania M  et al. Identification of thyroid tumor cell vulnerabilities through a siRNA-based functional screening. Oncotarget 6:34629-48 (2015). WB . Read more (PubMed: 26431489) »
  • Chen YL  et al. ENSA expression correlates with attenuated tumor propagation in liver cancer. Biochem Biophys Res Commun 442:56-61 (2013). IP ; Human . Read more (PubMed: 24211627) »

See all 3 Publications for this product

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