製品の概要

  • 製品名Anti-Mast Cell Tryptase antibody [AA1]
    Mast Cell Tryptase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AA1] to Mast Cell Tryptase
  • 特異性This antibody reacts with mast cells distributed in skin, synovium, lung and heart. It does not bind with any other cell type. Recognises human mast cell tryptase, both alpha and beta isoforms
  • アプリケーション適用あり: ICC, WB, IHC-P, ICC/IF, IHC-R, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cat, Dog, Monkey
  • 免疫原

    Purified human lung Tryptase

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following human tissue lysates: Lung; Tonsil; Skin.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 35-37 kDa (predicted molecular weight: 31 kDa).

We recommend using 3% milk as the blocking agent for Western blot.

IHC-P Use a concentration of 0.1 µg/ml.
ICC/IF 1/100.
IHC-R Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 関連性Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation.
  • 細胞内局在Secreted. Note: released from the secretory granules upon mast cell activation.
  • 参照データベース
  • 別名
    • alpha II antibody
    • Lung tryptase antibody
    • Mast cell alpha II tryptase antibody
    • Mast cell beta I tryptase antibody
    • Mast cell protease 7 antibody
    • Mast cell protease II antibody
    • MCP 7 antibody
    • Pituitary tryptase antibody
    • Skin tryptase antibody
    • TPS 1 antibody
    • TPS1 antibody
    • TPS2 antibody
    • TPSAB1 antibody
    • TPSAB1 protein antibody
    • TPSB1 antibody
    • Tryptase 1 antibody
    • Tryptase alpha 1 antibody
    • tryptase alpha I included antibody
    • Tryptase alpha II antibody
    • tryptase alpha II included antibody
    • tryptase alpha included antibody
    • tryptase alpha/beta 1 antibody
    • Tryptase beta 1 antibody
    • tryptase beta I included antibody
    • Tryptase I antibody
    • tryptase I included antibody
    • Tryptase III antibody
    • Tryptase skin antibody
    see all

Anti-Mast Cell Tryptase antibody [AA1] 画像

  • All lanes : Anti-Mast Cell Tryptase antibody [AA1] (ab2378) at 5 µg/ml

    Lane 1 : Human lung normal tissue lysate - total protein (40 - 65 years) (ab30281)
    Lane 2 : Human tonsil normal tissue lysate - total protein (ab29615)
    Lane 3 : Human skin tissue lysate - total protein (ab30166)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 35-37 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

    Mast Cell Tryptase contains an 18-residue signal sequence and a 12-residue activation peptide. The protein also has two potential glycosylation sites (Swissprot data). These post-translational modifications might explain the banding pattern observed. Abcam used 5% milk in TBS-T as a blocking agent for this blot. We found that this blocking agent removed more non-specific bands than BSA.

  • ab2378 staining Mast Cell Tryptase in Human Tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/7500 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • ab2378 staining Mast Cell Tryptase in Human nasal polyp and tonsil tissue sections by Immunohistochemistry (resin-embedded sections). Tissue was fixed with acetone and inhibitors and embedded in GMA resin. Samples were incubated with primary antibody (1/2000 in Tris buffered saline) for 18 hours at 21°C. A Biotin-conjugated Rabbit anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab2378 staining Mast Cell Tryptase in Mouse RAW 264.7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% Super block for 1 hour at 22°C. Samples were incubated with primary antibody (1/150 in PBS + 10% super block) for 14 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody

    See Abreview

  • ab2378 staining Mast Cell Tryptase in Rat Lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab2378 staining Mast Cell Tryptase in Mouse Lung (LPS inflammation) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 4% M.O.M Ig blocking for 1 hour at 20°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/200 in blocking buffer) for 16 hours at 4°C. A Biotin-conjugated Rabbit anti-mouse IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

  • ab2378 staining formalin fixed paraffin-embedded human colon tissue sections cut at 1 micron.  The section was subjected to heat mediated antigen retrieval in Dako Target Retrieval Solution and permeabilized in Triton-X prior to blocking in 6% BSA for 2 hours at 24°C.  The primary anitbody was diluted 1/250 and incubated with the sample for 16 hours at 5°C.  The secondary antibody was ab7064 (diluted 1/2000) and the slide was counterstained with DAPI.

    See Abreview

  • ab2378 staining Mast Cell Tryptase in Human mast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with Saponin 0.1% in PBS and blocked with 4% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in blocking buffer) for 14 hours at 4°C. A Texas Red®-conjugated Goat anti-mouse polyclonal (1/100) was used as the secondary antibody.

    See Abreview

Anti-Mast Cell Tryptase antibody [AA1] (ab2378) 使用論文

This product has been referenced in:
  • Maywald RL  et al. IL-33 activates tumor stroma to promote intestinal polyposis. Proc Natl Acad Sci U S A 112:E2487-96 (2015). Read more (PubMed: 25918379) »
  • De Martin S  et al. Expression and distribution of the adrenomedullin system in newborn human thymus. PLoS One 9:e97592 (2014). IHC-P ; Human . Read more (PubMed: 24831942) »

See all 14 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (nasal mucosa)
Permeabilization No
Specification nasal mucosa
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Mr. Duc Dung Le

Verified customer

投稿 Jun 09 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application IHC - Wholemount
Sample Mouse Tissue (Colon)
Specification Colon
Username

Abcam user community

Verified customer

投稿 May 04 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung Biopsy)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer, ph=6
Permeabilization No
Specification Lung Biopsy
Fixative Formaldehyde
Username

Dr. Francois Daubeuf

Verified customer

投稿 Apr 28 2015

Application Western blot
Sample Mouse Cell lysate - whole cell (Mature (8weeks) and immature BMMCs)
Gel Running Conditions Reduced Denaturing (Precast Gel 4-12%, Criterion TGX (Biorad))
Loading amount 2 µg
Treatment Cells were mixed in Rippa buffer and denaturated in Laemmli buffer 5min à 95°C
Specification Mature (8weeks) and immature BMMCs
Blocking step Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Dr. Francois Daubeuf

Verified customer

投稿 Apr 22 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Cat Tissue sections (Colon)
Specification Colon
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Aug 22 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Goat Tissue sections (Colon)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Colon
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Aug 22 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Chicken Tissue sections (Small intestine)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Small intestine
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Aug 22 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Dog Tissue sections (Colon)
Specification Colon
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Aug 22 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Human Tissue sections (Tonsil)
Specification Tonsil
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Aug 22 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Skin)
Permeabilization Yes - 0,3% Triton X
Specification Skin
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative Perfusion fixed with paraformaldehyde and picric acid
Username

Abcam user community

Verified customer

投稿 Aug 19 2014

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