Anti-MASH1/Achaete-scute homolog 1 抗体 (ab74065)

製品の概要

  • 製品名Anti-MASH1/Achaete-scute homolog 1 antibody
    MASH1/Achaete-scute homolog 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MASH1/Achaete-scute homolog 1
  • 特異性Ab74065 has been batch tested in WB and IF only. Some customers have successfully used this product in IHC. Please contact Abcam Scientific Support for more information.
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Zebrafish
    交差が予測される動物種: Chicken, Dog, Xenopus laevis, Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human MASH1/Achaete-scute homolog 1.

    (Peptide available as ab74622.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: DU 145 Whole Cell Rat Brain Tissue Mouse Brain Tissue IF/ICC: SKNSH cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab74065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-P 1/1250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能May play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Activates transcription by binding to the E box (5'-CANNTG-3').
  • 配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Achaete scute complex homolog 1 antibody
    • Achaete scute complex homolog like 1 antibody
    • Achaete scute complex homologue 1 antibody
    • Achaete scute complex homologue like 1 antibody
    • Achaete scute complex like 1 antibody
    • Achaete scute protein antibody
    • Achaete-scute homolog 1 antibody
    • Ascl 1 antibody
    • ascl1 antibody
    • ASCL1_HUMAN antibody
    • Ash 1 antibody
    • ASH-1 antibody
    • Ash1 antibody
    • bHLHa46 antibody
    • Class A basic helix-loop-helix protein 46 antibody
    • Hash 1 antibody
    • HASH1 antibody
    • Mammalian achaete scute homolog 1 antibody
    • Mammalian achaete scute homologue 1 antibody
    • Mash 1 antibody
    • Mash1 antibody
    see all

Anti-MASH1/Achaete-scute homolog 1 antibody 画像

  • All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065) at 1 µg/ml

    Lane 1 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size : 25 kDa
    Observed band size : 25,28 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab74065 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74065, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC-P image of MASH1 staining on mouse SVZ sections using ab74065 (1:250). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid pH 6. The sections were then blocked using 1% BSA solution at 21°C for 10 mins. The primary antibiody was incubated at 21°C for 2 hours.

    See Abreview

  • All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065) at 1 µg/ml

    Lane 1 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 25 kDa


    Exposure time : 3 minutes
  • All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Mouse brain homogenate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes
  • IHC-P image of MASH1 staining on rat SVZ sections using ab74065 (1:1250). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid pH 6. The sections were then blocked using 1% BSA solution at 21°C for 10 mins. The primary antibiody was incubated at 21°C for 2 hours.

    See Abreview

Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065) 使用論文

This product has been referenced in:
  • Andersen J  et al. A transcriptional mechanism integrating inputs from extracellular signals to activate hippocampal stem cells. Neuron 83:1085-97 (2014). ChIP ; Mouse . Read more (PubMed: 25189209) »
  • Guo X  et al. Derivation of sensory neurons and neural crest stem cells from human neural progenitor hNP1. Biomaterials 34:4418-27 (2013). ICC/IF ; Human . Read more (PubMed: 23498896) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer - tween 0,05 ph6
Sample Chicken Tissue sections (Transverse section of chick embryos (spinal cord))
Specification Transverse section of chick embryos (spinal cord)
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 16 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse brain)
Specification Mouse brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step (agent) for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Oct 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Rat Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Sep 17 2012

Es freut mich zu hören, dass Sie unser Angebot unseren ab74065 Anti-MASH1/Achaete-scute homolog 1 Antikörper in IHC zu testen, annehmen möchten. Wie besprochen können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns e...

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Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step None
Permeabilization No
Specification Brain
Fixative Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

投稿 Jun 08 2012

DISCOUNT CODE: **********Expiration date: ********* I am very pleased to hear you would like to accept our offer and test ab74065 in ChIP. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit a...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

投稿 Sep 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"