製品の概要

  • 製品名Anti-MARVELD2 antibody
    MARVELD2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MARVELD2
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • 免疫原

    Synthetic peptide corresponding to a region between residue 90 and 140 of human MARVELD2 (NP_001033692.1).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 - 8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 64 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性Tight junctions (TJ) prevent leakage of solutes through the paracellular pathway of epithelial cells. MARVELD2, or tricellulin (TRIC), is an integral membrane protein concentrated at the vertically oriented TJ strands of tricellular contacts. It plays a role in the formation of the epithelial barriers.
    • 細胞内局在Cell membrane; Multi-pass membrane protein. Cell junction, tight junction. Note=Found at tricellular contacts.
    • 参照データベース
    • 別名
      • DFNB49 antibody
      • FLJ30532 antibody
      • MARVD2 antibody
      • MARVEL (membrane associating) domain containing 2 antibody
      • MARVEL domain containing 2 antibody
      • MARVEL domain containing protein 2 antibody
      • MARVELD 2 antibody
      • MARVELD2 antibody
      • MRVLDC2 antibody
      • Tric antibody
      • Tricellulin antibody
      see all

    Anti-MARVELD2 antibody 画像

    • All lanes : Anti-MARVELD2 antibody (ab80421) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg

      Developed using the ECL technique

      Predicted band size : 64 kDa
      Observed band size : 64 kDa


      Exposure time : 10 minutes
    • Detection of human MARVELD2 in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab80421 at 3 µg/mg lysate for IP (lane 1). For subsequent WB detection, 1 µg/ml of ab80421 was used. Lane 2 represents control IgG IP.
      Detection: chemiluminescence with an exposure time of 10 seconds.

    Anti-MARVELD2 antibody (ab80421) 使用論文

    This product has been referenced in:
    • Masuda R  et al. Negative regulation of the tight junction protein tricellulin by snail-induced epithelial-mesenchymal transition in gastric carcinoma cells. Pathobiology 77:106-13 (2010). Read more (PubMed: 20332670) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab80421.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"