Anti-MARK 1+2+3+4 抗体 (ab74131)
Key features and details
- Rabbit polyclonal to MARK 1+2+3+4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG
製品の概要
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製品名
Anti-MARK 1+2+3+4 antibody -
製品の詳細
Rabbit polyclonal to MARK 1+2+3+4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human, African green monkey
交差が予測される動物種: Rat -
免疫原
Synthetic non-phosphopeptide derived from human MARK1/2/3/4 around the phosphorylation site of threonine 215 (L-D-TP-F-C).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74131の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 89 kDa.
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/100 - 1/500.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 89 kDa. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/500. |
ターゲット情報
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関連性
This complex of proteins may play a role in cytoskeletal stability, the control of cell polarity, microtubule stability and cancer. -
細胞内局在
MARK1: Cytoplasmic, cytoskeleton. MARK2: Cell membrane; Peripheral membrane protein. -
参照データベース
- Entrez Gene: 2011 Human
- Entrez Gene: 4139 Human
- Entrez Gene: 4140 Human
- Entrez Gene: 57787 Human
- Entrez Gene: 117016 Rat
- Entrez Gene: 170577 Rat
- Entrez Gene: 60328 Rat
- Entrez Gene: 680407 Rat
see all -
別名
- MAP/microtubule affinity regulating kinase 1 antibody
- MAP/microtubule affinity regulating kinase 2 antibody
- MAP/microtubule affinity regulating kinase 3 antibody
see all
画像
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All lanes : Anti-MARK 1+2+3+4 antibody (ab74131) at 1/500 dilution
Lane 1 : Extracts from COS-7 cells
Lane 2 : Extracts from COS-7 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 89 kDa
Observed band size: 89 kDa -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab74131, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
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Immunofluorescence analysis of HeLa cells, using ab74131 at 1/100 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab74131 は 3 報の論文で使用されています。
- Timilshina M et al. Activation of Mevalonate Pathway via LKB1 Is Essential for Stability of Treg Cells. Cell Rep 27:2948-2961.e7 (2019). PubMed: 31167140
- Tang EI et al. Microtubule affinity-regulating kinase 4 (MARK4) is a component of the ectoplasmic specialization in the rat testis. Spermatogenesis 2:117-126 (2012). PubMed: 22670221
- Mian I et al. LKB1 destabilizes microtubules in myoblasts and contributes to myoblast differentiation. PLoS One 7:e31583 (2012). WB ; Mouse . PubMed: 22348111