Anti-MARCKS (phospho S152 + S156) 抗体 (ab32590)
Key features and details
- Rabbit polyclonal to MARCKS (phospho S152 + S156)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-MARCKS (phospho S152 + S156) antibody
MARCKS 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MARCKS (phospho S152 + S156) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat -
免疫原
Synthetic peptide corresponding to MARCKS (phospho S152 + S156).
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ポジティブ・コントロール
- Rat brain lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32590の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 31 kDa).
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特記事項 |
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WB
1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 31 kDa). |
ターゲット情報
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機能
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. -
配列類似性
Belongs to the MARCKS family. -
翻訳後修飾
Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity. -
細胞内局在
Cytoplasm > cytoskeleton. Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 25603 Rat
- SwissProt: P30009 Rat
- Unigene: 9560 Rat
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別名
- 80 kDa protein antibody
- 80K L antibody
- 80K L protein antibody
see all
画像
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All lanes : Anti-MARCKS (phospho S152 + S156) antibody (ab32590) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat brain lysate with Ser152/156 phosphopeptide used as antigen
Lysates/proteins at 10 µg per lane.
Predicted band size: 31 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?
The corresponding dephosphopeptide did not block the immunolabeling (not shown).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab32590 は 1 報の論文で使用されています。
- Kim JH et al. Intramyocardial Adipose-Derived Stem Cell Transplantation Increases Pericardial Fat with Recovery of Myocardial Function after Acute Myocardial Infarction. PLoS One 11:e0158067 (2016). Flow Cyt . PubMed: 27336402