製品の概要

  • 製品名Anti-MARCKS antibody
    MARCKS 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MARCKS
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Cow, Human
    交差が予測される動物種: all Mammals
  • 免疫原

    Recombinant full length rat MARCKS expressed in and purified from E. coli.

  • ポジティブ・コントロール
    • rat cerebral cortex lysate, Mixed neuron/glial cultures.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 30 kDa).
ICC/IF 1/500.

ターゲット情報

  • 機能MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
  • 配列類似性Belongs to the MARCKS family.
  • 翻訳後修飾Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
  • 細胞内局在Cytoplasm > cytoskeleton. Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 80 kDa protein antibody
    • 80K L antibody
    • 80K L protein antibody
    • 80K-L protein antibody
    • 80KL antibody
    • 81 kDa protein, light chain antibody
    • light chain antibody
    • MACS antibody
    • MARCKS antibody
    • MARCS antibody
    • MARCS_HUMAN antibody
    • MGC52672 antibody
    • myristoylated alanine rich C kinase substrate antibody
    • Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L) antibody
    • Myristoylated alanine rich protein kinase C substrate antibody
    • Myristoylated alanine-rich C-kinase substrate antibody
    • Phosphomyristin antibody
    • PKCSL antibody
    • PRKCSL antibody
    • protein kinase C substrate 80 kDa protein light chain antibody
    • Protein kinase C substrate antibody
    see all

Anti-MARCKS antibody 画像

  • Anti-MARCKS antibody (ab72459) at 1/10000 dilution + rat cerebral cortex lysate

    Predicted band size : 30 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • Mixed neuron/glial cultures stained with MARCKS antibody ab72459 at 1/500 (red), and with an anti-MAP2 antibody (green).

Anti-MARCKS antibody (ab72459) 使用論文

ab72459 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Oligodendrocyte)
Loading amount 30 µg
Specification Oligodendrocyte
Gel Running Conditions Reduced Denaturing (12)
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

投稿 May 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Oligodendrocyte)
Specification Oligodendrocyte
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

投稿 May 12 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"