製品の概要
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製品名Anti-MARCKS antibody
MARCKS 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to MARCKS
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由来種Rabbit
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特異性ab51100 detects endogenous levels of total MARCKS protein.
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アプリケーション適用あり: WB, IHC-P, ELISAmore details
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種交差性交差種: Mouse, Rat, Human
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免疫原
A synthentic non-phosphopeptide derived from human MARCKS around the phosphorylation site of serine 162 (K-K-SP-F-K).
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ポジティブ･コントロール
- Human brain tissue
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）ab51100 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Corresponding phospho antibody
アプリケーション
Our Abpromise guarantee covers the use of ab51100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/2000. Incubate for 1 hour. | |
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | 1/40000. |
ターゲット情報
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機能MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
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配列類似性Belongs to the MARCKS family.
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翻訳後修飾Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
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細胞内局在Cytoplasm > cytoskeleton. Membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 4082 Human
- Entrez Gene: 17118 Mouse
- Entrez Gene: 25603 Rat
- Omim: 177061 Human
- SwissProt: P29966 Human
- SwissProt: P26645 Mouse
- SwissProt: P30009 Rat
- Unigene: 519909 Human
see all -
別名
- 80 kDa protein antibody
- 80K L antibody
- 80K L protein antibody
see all
画像
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Immunohistochemistry (Paraffin-embedded sections) - MARCKS antibody (ab51100)ab51100 at 1/100 dilution staining MARCKS in paraffin embedded human brain sections by Immunohistochemistry, in the absence and presence of the immunizing (blocking) peptide. -
Western blot - MARCKS antibody (ab51100)This image is courtesy of an anonymous AbreviewAnti-MARCKS antibody (ab51100) at 1/2000 dilution + 293 cells, lysed in RIPA buffer containing minicomplete protease inhibitors at 30 µg
Secondary
HRP conjugated goat anti-rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 63 kDa (why is the actual band size different from the predicted?)
Additional bands at: 19 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Primary antibody was incubated with the blot for 1 hour at 20°C. Run on neutral pH gel which may account for unexpectedly low MW due to charge effects.
参考文献
This product has been referenced in:
- Johnston DGW et al. MicroRNA-21 Limits Uptake of Listeria monocytogenes by Macrophages to Reduce the Intracellular Niche and Control Infection. Front Cell Infect Microbiol 7:201 (2017). Read more (PubMed: 28589100) »
- Qi X et al. Cathepsin B modulates lysosomal biogenesis and host defense against Francisella novicida infection. J Exp Med 213:2081-97 (2016). WB . Read more (PubMed: 27551156) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"