製品の概要

  • 製品名Anti-MARCH-I antibody
    MARCH-I 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MARCH-I
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (LQASSPTTGT APRSQSRLSV CPSTQDICRI CHCEGDEESP LITPCRCTGT) of human MARCH-I (NP_060393).

  • ポジティブ・コントロール
    • Fetal muscle lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, downregulates their cell surface localization thus sequestering them in the intracellular endosomal system.
  • 組織特異性Expressed in antigen presenting cells, APCs, located in lymph nodes and spleen. Also expressed in lung. Expression is high in follicular B cells, moderate in dendritic cells and low in splenic T cells.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Contains 1 RING-CH-type zinc finger.
  • 発生段階During maturation of dendritic cells, expression is down-regulated and stabilizes MHC class II proteins accumulate at the plasma membrane.
  • ドメインThe RING-CH-type zinc finger domain is required for E3 ligase activity.
  • 翻訳後修飾Has a short half-life. Instability/short half-life permits rapid changes that allow efficient induction of antigen presentation once antigen presenting cells, APCs, receive maturation signals. Small changes in protein levels significantly alter the cell surface display of MHC class II proteins.
  • 細胞内局在Golgi apparatus > trans-Golgi network membrane. Lysosome membrane. Cytoplasmic vesicle membrane. Late endosome membrane. Early endosome membrane. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp564M1682 antibody
    • E3 ubiquitin protein ligase MARCH1 antibody
    • E3 ubiquitin-protein ligase MARCH1 antibody
    • FLJ20668 antibody
    • MARCH 1 antibody
    • MARCH-I antibody
    • March1 antibody
    • MARH1_HUMAN antibody
    • Membrane associated RING CH protein I antibody
    • Membrane associated ring finger (C3HC4) 1 antibody
    • Membrane associated ring finger (C3HC4) 1, E3 ubiquitin protein ligase antibody
    • Membrane associated RING finger protein 1 antibody
    • Membrane-associated RING finger protein 1 antibody
    • Membrane-associated RING-CH protein I antibody
    • RING finger protein 171 antibody
    • RNF171 antibody
    see all

Anti-MARCH-I antibody 画像

  • Anti-MARCH-I antibody (ab83469) at 1 µg/ml + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 31 kDa
    Observed band size : 31 kDa

Anti-MARCH-I antibody (ab83469) 使用論文

ab83469 has not yet been referenced specifically in any publications.

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