製品の概要

  • 製品名
    Anti-MAPRE1 antibody [KT51]
    MAPRE1 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [KT51] to MAPRE1
  • アプリケーション
    適用あり: IP, ELISA, WB, ICC/IF, Flow Cyt, ICCmore details
  • 種交差性
    交差種: Mouse, Hamster, Human
  • 免疫原

    The C terminal part of Mouse MAPRE1 (amino acids 125-268) fused to a tag, purified from E. coli BL21 bacteria.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use a concentration of 2 µg/ml.
ELISA Use at an assay dependent concentration.
WB 1/500 - 1/7000. Predicted molecular weight: 30 kDa.
ICC/IF 1/100 - 1/1000.
Flow Cyt Use 1µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC Use at an assay dependent concentration.

ターゲット情報

  • 機能
    May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Belongs to the MAPRE family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 EB1 C-terminal domain.
  • ドメイン
    Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
  • 細胞内局在
    Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Associated with the microtubule network at the growing distal tip of microtubules. Also enriched at the centrosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5530600P05Rik antibody
    • Adenomatous polyposis coli binding protein EB 1 antibody
    • Adenomatous polyposis coli binding protein EB1 antibody
    • AI462499 antibody
    • AI504412 antibody
    • APC binding protein EB 1 antibody
    • APC binding protein EB1 antibody
    • APC-binding protein EB1 antibody
    • AW260097 antibody
    • BIM1p antibody
    • D2Ertd459e antibody
    • EB 1 antibody
    • EB1 antibody
    • End binding protein 1 antibody
    • End-binding protein 1 antibody
    • fa01e12 antibody
    • fc23e11 antibody
    • fi33c06 antibody
    • MAPRE 1 antibody
    • MAPRE1 antibody
    • Mapre3 antibody
    • MARE1_HUMAN antibody
    • MGC117374 antibody
    • MGC129946 antibody
    • MGC52508 antibody
    • Microtubule associated protein RP/EB family member 1 antibody
    • Microtubule-associated protein RP/EB family member 1 antibody
    • wu:fa01e12 antibody
    • wu:fc23e11 antibody
    • wu:fi33c06 antibody
    • zgc:55428 antibody
    • zgc:77807 antibody
    • zgc:85755 antibody
    see all

Anti-MAPRE1 antibody [KT51] 画像

  • ab53358 staining MAPRE1 in Hela cells (red). Cells were fixed in methanol, permeabilised with 0.5% Triton X100/ PBS and counterstained both with DAPI - to highlight the nucleus (blue) - and an anti-tubulin antibody (green). Please refer to abreview for further experimental details.

    See Abreview

  • Anti-MAPRE1 antibody [KT51] (ab53358) at 1/1000 dilution + lysate prepared from HeLa cells at 50 µg

    Secondary
    Rabbit Anti-Rat IgG H&L (HRP) (ab6734) at 1/2000 dilution
    Developed using the ECL technique

    Predicted band size : 30 kDa


    Exposure time : 5 seconds

    Image courtesy of an anonymous Abreview.

Anti-MAPRE1 antibody [KT51] (ab53358) 使用論文

This product has been referenced in:
  • Thomas GE  et al. EB1 regulates attachment of Ska1 with microtubules by forming extended structures on the microtubule lattice. Nat Commun 7:11665 (2016). Read more (PubMed: 27225956) »
  • Chinen T  et al. The ?-tubulin-specific inhibitor gatastatin reveals temporal requirements of microtubule nucleation during the cell cycle. Nat Commun 6:8722 (2015). Read more (PubMed: 26503935) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (MEF)
Loading amount
50 µg
Specification
MEF
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Feb 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (HELA)
Specification
HELA
Fixative
Methanol
Permeabilization
Yes - 0.5% Triton-X
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Feb 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (HELA)
Loading amount
50 µg
Specification
HELA
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Feb 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

投稿 Nov 05 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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