製品の概要

  • 製品名Anti-MAPRE1 antibody
    MAPRE1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MAPRE1
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Chicken, Cow, Pig, Non Human Primates
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human MAPRE1.

    (Peptide available as ab73305.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Thymus (Rat) Tissue Jurkat Whole Cell Ramos Whole Cell TE 671 Whole Cell HeLa Whole Cell

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 30 kDa).
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
  • 組織特異性Ubiquitously expressed.
  • 配列類似性Belongs to the MAPRE family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 EB1 C-terminal domain.
  • ドメインComposed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
  • 細胞内局在Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Associated with the microtubule network at the growing distal tip of microtubules. Also enriched at the centrosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5530600P05Rik antibody
    • Adenomatous polyposis coli binding protein EB 1 antibody
    • Adenomatous polyposis coli binding protein EB1 antibody
    • AI462499 antibody
    • AI504412 antibody
    • APC binding protein EB 1 antibody
    • APC binding protein EB1 antibody
    • APC-binding protein EB1 antibody
    • AW260097 antibody
    • BIM1p antibody
    • D2Ertd459e antibody
    • EB 1 antibody
    • EB1 antibody
    • End binding protein 1 antibody
    • End-binding protein 1 antibody
    • fa01e12 antibody
    • fc23e11 antibody
    • fi33c06 antibody
    • MAPRE 1 antibody
    • MAPRE1 antibody
    • Mapre3 antibody
    • MARE1_HUMAN antibody
    • MGC117374 antibody
    • MGC129946 antibody
    • MGC52508 antibody
    • Microtubule associated protein RP/EB family member 1 antibody
    • Microtubule-associated protein RP/EB family member 1 antibody
    • wu:fa01e12 antibody
    • wu:fc23e11 antibody
    • wu:fi33c06 antibody
    • zgc:55428 antibody
    • zgc:77807 antibody
    • zgc:85755 antibody
    see all

Anti-MAPRE1 antibody 画像

  • All lanes : Anti-MAPRE1 antibody (ab66105) at 1 µg/ml

    Lane 1 : Thymus (Rat) Tissue Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 4 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 53 kDa (possible post-translational modification).
  • ICC/IF image of ab66105 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66105, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 1µg/ml.

Anti-MAPRE1 antibody (ab66105) 使用論文

ab66105 has not yet been referenced specifically in any publications.

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