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RabMAb

Anti-MAPKAP Kinase 2 抗体 [E341] (ab32567)

製品の概要

  • 製品名
    Anti-MAPKAP Kinase 2 antibody [E341]
    MAPKAP Kinase 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E341] to MAPKAP Kinase 2
  • 由来種
    Rabbit
  • 特異性
    ab32567 recognises MAPKAP Kinase-2.
  • アプリケーション
    適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC or IHC
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near C-terminus of human MAPKKAP Kinase-2.

  • ポジティブ・コントロール
    • Hela cell lysate.
  • 特記事項

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
IP 1/200.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC or IHC.
  • ターゲット情報

    • 機能
      Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
    • 組織特異性
      Expressed in all tissues examined.
    • 配列類似性
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.
    • 翻訳後修飾
      Phosphorylated and activated by MAP kinase.
    • Information by UniProt
    • 参照データベース
    • 別名
      • MAP kinase activated protein Kinase 2 antibody
      • MAP kinase-activated protein kinase 2 antibody
      • MAPK activated protein kinase 2 antibody
      • MAPK-activated protein kinase 2 antibody
      • MAPK2_HUMAN antibody
      • MAPKAP K2 antibody
      • MAPKAP kinase 2 antibody
      • MAPKAPK 2 antibody
      • MAPKAPK-2 antibody
      • MAPKAPK2 antibody
      • Mitogen activated protein kinase activated protein kinase 2 antibody
      • MK 2 antibody
      • MK2 antibody
      see all

    画像

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MAPKAP Kinase 2knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: A431 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab32567 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32567 was shown to specifically react with MAPKAP Kinase 2 when MAPKAP Kinase 2 knockout samples were used. Wild-type and MAPKAP Kinase 2 knockout samples were subjected to SDS-PAGE. ab32567 and ab8245 (loading control to ProteinX2) were diluted to 1/500 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Anti-MAPKAP Kinase 2 antibody [E341] (ab32567) at 1/1000 dilution + Hela cell lysate

      Predicted band size: 46 kDa
      Observed band size: 46 kDa

    • Lanes 1-2 : Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018) at 1/500 dilution
      Lanes 3-4 : Anti-MAPKAP Kinase 2 antibody [E341] (ab32567) at 1/500 dilution

      Lanes 1 & 3 : UV treated HeLa cells
      Lanes 2 & 4 : UV treated HeLa cells + p38 inhibitor

      Secondary
      All lanes : IRDye® 680 LICOR goat anti-rabbit.

      Performed under reducing conditions.

      Predicted band size: 46 kDa
      Observed band size: 48 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 55 kDa (possible cross reactivity)


      Exposure time: 1 minute


      ab32567 recognise total MK2 (lane 3,4). A control for specificity is shown as UV treated HeLa + p38 inhibitor (lane 2,4). While the intensity of pT334 band is greatly reduced (lane 2), total MK2 remains unaltered (lane 4).

      See Abreview

    参考文献

    This product has been referenced in:
    • McCaskill JL  et al. Broad-Spectrum Inhibition of Respiratory Virus Infection by MicroRNA Mimics Targeting p38 MAPK Signaling. Mol Ther Nucleic Acids 7:256-266 (2017). Dog, Human . Read more (PubMed: 28624201) »

    See 1 Publication for this product

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Loading amount
    35 µg
    Specification
    HeLa cells
    Treatment
    UV treated samples (20 J dose)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    投稿 Aug 20 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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