製品の概要

  • 製品名Anti-MAPK6 antibody [EP1720Y]
    MAPK6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1720Y] to MAPK6
  • アプリケーション適用あり: IP, WB, Flow Cyt, IHC-Pmore details
    適用なし: ICC/IF
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human MAPK6 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q16659

  • ポジティブ・コントロール
    • HeLa cell lysate or human breast carcinoma tissue.
  • 特記事項

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration. PubMed: 22505454
WB 1/1000 - 1/10000. Detects a band of approximately 105 kDa (predicted molecular weight: 63 kDa).
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for ICC/IF.
  • ターゲット情報

    • 機能Phosphorylates microtubule-associated protein 2 (MAP2). May promote entry in the cell cycle.
    • 組織特異性Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.
    • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • ドメインThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • 翻訳後修飾Dually phosphorylated on Thr-626 and Tyr-628, which activates the enzyme.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ERK-3 antibody
      • ERK3 antibody
      • Extracellular signal regulated kinase 3 antibody
      • Extracellular signal regulated kinase p97 antibody
      • Extracellular signal-regulated kinase 3 antibody
      • HsT17250 antibody
      • MAP kinase 6 antibody
      • MAP kinase isoform p97 antibody
      • MAPK 6 antibody
      • MAPK6 antibody
      • Mitogen activated protein kinase 6 antibody
      • Mitogen-activated protein kinase 6 antibody
      • MK06_HUMAN antibody
      • p97 MAPK antibody
      • p97-MAPK antibody
      • PRKM6 antibody
      • Protein kinase mitogen activated 5 antibody
      • Protein kinase mitogen activated 6 antibody
      see all

    Anti-MAPK6 antibody [EP1720Y] 画像

    • Anti-MAPK6 antibody [EP1720Y] (ab53277) at 1/1000 dilution + HeLa cell lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 63 kDa
      Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    • Overlay histogram showing A431 cells stained with ab53277 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53277, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Ab53277 (1:50) staining human MAPK6 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.

    Anti-MAPK6 antibody [EP1720Y] (ab53277) 使用論文

    This product has been referenced in:

    See all 4 Publications for this product

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