製品の概要

  • 製品名Anti-MAPK4 antibody
    MAPK4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MAPK4
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Cow
  • 免疫原

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 296 of MAPK4 (NP_002738).

  • ポジティブ・コントロール
    • WB: H1299 whole cell lysate; A431, HelaS3, HepG2, MOLT4 and Raji cell lysates ICC/IF: HeLa cell

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 66 kDa.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle.
  • 組織特異性High expression in heart and brain.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • ドメインThe FRIEDE motif is required for docking MAPKAPK5.
    In contrast to classical MAPKs, the TXY motif within the activation loop is replaced by the SEG motif, whose phosphorylation activates the MAP kinases.
  • 翻訳後修飾Phosphorylated at Ser-186 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites.
  • 細胞内局在Cytoplasm. Nucleus. Translocates to the cytoplasm following interaction with MAPKAPK5.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ERK-4 antibody
    • ERK3 antibody
    • Erk3 related antibody
    • ERK4 antibody
    • Extracellular signal regulated kinase 4 antibody
    • Extracellular signal-regulated kinase 4 antibody
    • MAP kinase 4 antibody
    • MAP kinase isoform p63 antibody
    • MAPK 4 antibody
    • MAPK4 antibody
    • Mitogen activated protein kinase 4 antibody
    • Mitogen-activated protein kinase 4 antibody
    • MK04_HUMAN antibody
    • p63 MAPK antibody
    • p63-MAPK antibody
    • p63MAPK antibody
    • PRKM4 antibody
    • Protein kinase, mitogen-activated, 4 antibody
    • Protein-tyrosine kinase ERK3-related antibody
    • RMNK2 antibody
    see all

Anti-MAPK4 antibody 画像

  • Anti-MAPK4 antibody (ab96816) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 66 kDa
  • Immunofluorescence analysis of MAPK4 in paraformaldehyde fixed HeLa, using ab96816 at a 1/200 dilution.
    The lower image was merged with DNA probe.

Anti-MAPK4 antibody (ab96816) 使用論文

ab96816 has not yet been referenced specifically in any publications.

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