The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Detects a band of approximately 60 kDa.
1/10 - 1/50.
In vitro, phosphorylates MBP.
Widely expressed with a maximal expression in lung and kidney.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.
The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteasomal degradation. The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro. Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase.
Western blot - MAPK15 antibody - N-terminal (ab70911)
ab70911, at a 1/10 dilution, staining human MAPK15 in breast carcinoma, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue. ab70911 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.