製品の概要

  • 製品名Anti-MAP4K4 antibody
    MAP4K4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MAP4K4
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 850 and 900 of human MAP4K4 (AAO32626.1).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 - 8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab80418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 142 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway.
    • 組織特異性Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
    • 配列類似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 CNH domain.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • FLH21957 antibody
      • FLJ10410 antibody
      • FLJ20373 antibody
      • FLJ90111 antibody
      • Hepatocyte progenitor kinase like/germinal center kinase like kinase antibody
      • HGK antibody
      • HPK/GCK like kinase antibody
      • HPK/GCK like kinase HGK antibody
      • HPK/GCK-like kinase HGK antibody
      • KIAA0687 antibody
      • M4K4_HUMAN antibody
      • MAP4K4 antibody
      • MAPK/ERK kinase kinase kinase 4 antibody
      • MEK kinase kinase 4 antibody
      • MEKKK 4 antibody
      • MEKKK4 antibody
      • Mitogen activated protein kinase kinase kinase kinase 4 antibody
      • Mitogen-activated protein kinase kinase kinase kinase 4 antibody
      • Nck interacting kinase antibody
      • Nck-interacting kinase antibody
      • NIK antibody
      see all

    Anti-MAP4K4 antibody 画像

    • All lanes : Anti-MAP4K4 antibody (ab80418) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg

      Developed using the ECL technique

      Predicted band size : 142 kDa
      Observed band size : 171 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes
    • Detection of Human MAP4K4 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab80418 at 3 µg/mg lysate for IP (lane 1). For subsequent WB detection, 1 µg/ml of ab80418 was used. Detection was by chemiluminescence with an exposure time of 30 seconds. Lane 2 represents control IgG IP.

    Anti-MAP4K4 antibody (ab80418) 使用論文

    This product has been referenced in:
    • Santhana Kumar K  et al. The Ser/Thr kinase MAP4K4 drives c-Met-induced motility and invasiveness in a cell-based model of SHH medulloblastoma. Springerplus 4:19 (2015). WB ; Human . Read more (PubMed: 25625039) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab80418.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"