製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MAP4K4
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 850 and 900 of human MAP4K4 (AAO32626.1).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 - 8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報
    IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 142 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway.
    • 組織特異性
      Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
    • 配列類似性
      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 CNH domain.
      Contains 1 protein kinase domain.
    • 翻訳後修飾
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • FLH21957 antibody
      • FLJ10410 antibody
      • FLJ20373 antibody
      • FLJ90111 antibody
      • Hepatocyte progenitor kinase like/germinal center kinase like kinase antibody
      • HGK antibody
      • HPK/GCK like kinase antibody
      • HPK/GCK like kinase HGK antibody
      • HPK/GCK-like kinase HGK antibody
      • KIAA0687 antibody
      • M4K4_HUMAN antibody
      • MAP4K4 antibody
      • MAPK/ERK kinase kinase kinase 4 antibody
      • MEK kinase kinase 4 antibody
      • MEKKK 4 antibody
      • MEKKK4 antibody
      • Mitogen activated protein kinase kinase kinase kinase 4 antibody
      • Mitogen-activated protein kinase kinase kinase kinase 4 antibody
      • Nck interacting kinase antibody
      • Nck-interacting kinase antibody
      • NIK antibody
      see all

    画像

    • All lanes : Anti-MAP4K4 antibody (ab80418) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg

      Developed using the ECL technique

      Predicted band size : 142 kDa
      Observed band size : 171 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes
    • Detection of Human MAP4K4 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab80418 at 3 µg/mg lysate for IP (lane 1). For subsequent WB detection, 1 µg/ml of ab80418 was used. Detection was by chemiluminescence with an exposure time of 30 seconds. Lane 2 represents control IgG IP.

    参考文献

    This product has been referenced in:
    • Santhana Kumar K  et al. The Ser/Thr kinase MAP4K4 drives c-Met-induced motility and invasiveness in a cell-based model of SHH medulloblastoma. Springerplus 4:19 (2015). WB ; Human . Read more (PubMed: 25625039) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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