Rabbit polyclonal to MAP4K4
Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 850 and 900 of human MAP4K4 (AAO32626.1).
Whole cell lysate from HeLa cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 - 8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2 - 5 µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 142 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway.
Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain. Contains 1 protein kinase domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Western blot - MAP4K4 antibody (ab80418)
All lanes :
Anti-MAP4K4 antibody (ab80418) at 0.1 µg/ml
Lane 1 :
Whole cell lysate from HeLa cells at 50 µg
Lane 2 :
Whole cell lysate from HeLa cells at 15 µg
Lane 3 :
Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size:
Observed band size:
171 kDa (
why is the actual band size different from the predicted?
Immunoprecipitation - MAP4K4 antibody (ab80418)
Detection of Human MAP4K4 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab80418 at 3 µg/mg lysate for IP (lane 1). For subsequent WB detection, 1 µg/ml of ab80418 was used. Detection was by chemiluminescence with an exposure time of 30 seconds. Lane 2 represents control IgG IP.
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