Anti-MAP4K1/HPK1 抗体 [EP630Y] (ab33910)
Key features and details
- Rabbit monoclonal [EP630Y] to MAP4K1/HPK1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
Anti-MAP4K1/HPK1 antibody [EP630Y]
MAP4K1/HPK1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP630Y] to MAP4K1/HPK1 -
由来種
Rabbit -
特異性
ab33910 recognises hematopoietic progenitor kinase 1 (HPK1).
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アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: ICC or ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Raji and Daudi (human Burkitt's lymphoma) whole cell lysates. IHC-P: Human liver tissue.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.09% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP630Y -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab33910の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000 - 1/20000. Predicted molecular weight: 97 kDa.
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IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/5000 - 1/20000. Predicted molecular weight: 97 kDa. |
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. -
組織特異性
Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain.
Contains 1 protein kinase domain. - Information by UniProt
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参照データベース
- Entrez Gene: 11184 Human
- Omim: 601983 Human
- SwissProt: Q92918 Human
- Unigene: 95424 Human
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別名
- Hematopoietic progenitor kinase 1 antibody
- Hematopoietic progenitor kinase antibody
- HPK1 antibody
see all
画像
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All lanes : Anti-MAP4K1/HPK1 antibody [EP630Y] (ab33910) at 1/5000 dilution
Lane 1 : Daudi (human Burkitt's lymphoma) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution
Predicted band size: 97 kDaBlocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
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ab33910 staining MAP4K1/HPK1 in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.
Negative control 1: PBS in place of primary antibody.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab33910 は 5 報の論文で使用されています。
- Lim S et al. Identification of the kinase STK25 as an upstream activator of LATS signaling. Nat Commun 10:1547 (2019). PubMed: 30948712
- Chen X et al. Comprehensive Gene Expression Analysis in NMIBC Using RNA-seq Reveals New Therapy Strategies. Front Oncol 9:523 (2019). PubMed: 31293967
- Chen Y et al. Circular RNA circAGO2 drives cancer progression through facilitating HuR-repressed functions of AGO2-miRNA complexes. Cell Death Differ 26:1346-1364 (2019). PubMed: 30341421
- Li Y et al. Ubiquitin-specific protease 4 inhibits breast cancer cell growth through the upregulation of PDCD4. Int J Mol Med 38:803-11 (2016). Human . PubMed: 27430936
- Palmer RD et al. Pediatric malignant germ cell tumors show characteristic transcriptome profiles. Cancer Res 68:4239-47 (2008). IHC-P ; Human . PubMed: 18519683