Rabbit polyclonal to MAP4K1
Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
Synthetic peptide corresponding to Human MAP4K1 aa 218-267 (N terminal).
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent concentration.
Titre using peptide based assay 1/312500.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 91 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation.
Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain.
Contains 1 protein kinase domain.
Information by UniProt
- Hematopoietic progenitor kinase 1 antibody
- Hematopoietic progenitor kinase antibody
- HPK1 antibody
Western blot - Anti-MAP4K1 antibody (ab76379)
Anti-MAP4K1 antibody (ab76379) at 1 µg/ml + 721_B cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
91 kDaObserved band size:
75 kDa (why is the actual band size different from the predicted?
)Additional bands at:
31 kDa. We are unsure as to the identity of these extra bands.
Gel concentration 12%
has not yet been referenced specifically in any publications.
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