製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MAP4K1
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide (Human)

  • ポジティブ・コントロール
    • 721_B cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1/312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 91 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation.
  • 組織特異性
    Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.
  • 配列類似性
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CNH domain.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Hematopoietic progenitor kinase 1 antibody
    • Hematopoietic progenitor kinase antibody
    • HPK1 antibody
    • Human Hematopoietic Progenitor Kinase antibody
    • M4K1_HUMAN antibody
    • Map4k1 antibody
    • MAPK/ERK kinase kinase kinase 1 antibody
    • MEK kinase kinase 1 antibody
    • MEKKK 1 antibody
    • Mitogen activated protein kinase kinase kinase kinase 1 antibody
    • Mitogen-activated protein kinase kinase kinase kinase 1 antibody
    see all

画像

  • Anti-MAP4K1 antibody (ab76379) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 91 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab76379 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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