Rabbit polyclonal to MAP3K9 - C-terminal
Mouse, Rat, Human
Human breast carcinoma tissue and rat testis tissue and A375 cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein G purified
ab71628 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/100 - 1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 118 kDa).
Activates the JUN N-terminal pathway.
Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. Contains 1 SH3 domain.
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site.
Information by UniProt
Western blot - MAP3K9 antibody - C-terminal (ab71628)
All lanes :
Anti-MAP3K9 antibody - C-terminal (ab71628) at 1/100 dilution
Lane 1 :
A375 cell lysate
Lane 2 :
rat testis tissue lysate
Lysates/proteins at 12.5 µg per lane.
Predicted band size:
Observed band size:
100 kDa (
why is the actual band size different from the predicted?
Additional bands at:
35 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAP3K9 antibody - C-terminal (ab71628)
ab71628 at 1/50 dilution, staining MAP3K9 in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
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