製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MAP3K15
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide within residues: LEQKTQELYH LQLKLKSNCI TENPAGPYGQ RTDKELIDWL RLQGADAKTI, corresponding to internal sequence amino acids 684-733 of Human MAP3K15 isoform 3 (NP_001001671)

  • ポジティブ・コントロール
    • Human fetal intestine lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab90038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 89 kDa.Can be blocked with MAP3K15 peptide (ab220248). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    May function in a signal transduction pathway that is activated by various cell stresses and leads to apoptosis.
  • 組織特異性
    Isoform 2 and isoform 3 are widely expressed. Isoform 2 highest levels are observed in fetal brain, and isoform 3 highest levels in pancreas, peripheral blood leukocytes, fetal brain and spleen.
  • 配列類似性
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apoptosis signal-regulating kinase 3 antibody
    • ASK3 antibody
    • bA723P2.3 antibody
    • FLJ16518 antibody
    • M3K15_HUMAN antibody
    • Map3k15 antibody
    • MAPK/ERK kinase kinase 15 antibody
    • MEK kinase 15 antibody
    • MEKK 15 antibody
    • Mitogen activated protein kinase kinase kinase 15 antibody
    • Mitogen-activated protein kinase kinase kinase 15 antibody
    • OTTHUMP00000183504 antibody
    see all

画像

  • Anti-MAP3K15 antibody (ab90038) at 1 µg/ml + Human fetal intestine lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 89 kDa
    Observed band size : 89 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab90038 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (HepG2)
Total protein in input
1000 µg
Specification
HepG2
Immuno-precipitation step
Protein A/G
Username

Dr. Avi Shtevi

Verified customer

投稿 Dec 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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