製品の概要

  • 製品名Anti-MAP3K13 antibody
    MAP3K13 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to MAP3K13
  • アプリケーション適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Chimpanzee, Orangutan
  • 免疫原

    Recombinant fragment: SEPDKGQAGP WGCCQADAYD PCLQCRPEQY GSLDIPSAEP VGRSPDLSKS PAHNPLLENA QSSEKTEENE FSGCRSESSL GTSHLGTPPA LPRKTRPLQK, corresponding to amino acids 711-810 of Human MAP3K13 (NP_004712) with a proprietary tag.

  • ポジティブ・コントロール
    • Partial tagged recombinant MAP3K13 protein. K-562 cell lysate. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: Panc-1

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab88482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 108 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Activates the JUN N-terminal pathway through activation of the MAP kinase kinase MAP2K7. Acts synergistically with PRDX3 to regulate the activation of NF-kappa-B in the cytosol. This activation is kinase-dependent and involves activating the IKK complex, the IKBKB-containing complex that phosphorylates inhibitors of NF-kappa-B.
  • 組織特異性Expressed in the adult brain, liver, placenta and pancreas, with expression strongest in the pancreas.
  • 配列類似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylated on serine and threonine residues.
  • 細胞内局在Cytoplasm. Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC 2.7.11.25 antibody
    • Leucine zipper bearing kinase antibody
    • Leucine zipper-bearing kinase antibody
    • LZK antibody
    • M3K13_HUMAN antibody
    • MAP3K13 antibody
    • MEKK13 antibody
    • MGC133196 antibody
    • Mitogen activated protein kinase kinase kinase 13 antibody
    • Mitogen-activated protein kinase kinase kinase 13 antibody
    • Mixed lineage kinase antibody
    • MLK antibody
    • OTTHUMP00000210397 antibody
    see all

Anti-MAP3K13 antibody 画像

  • ICC/IF image of ab88482 stained Panc-1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab88482 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- mouse (ab150117) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-MAP3K13 antibody (ab88482) at 5 µg/ml + Partial tagged recombinant MAP3K13 protein (immmunogen) at 0.2 µg

    Predicted band size : 108 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 37 kDa.
  • Anti-MAP3K13 antibody (ab88482) at 5 µg/ml + K-562 cell lysate at 50 µg

    Predicted band size : 108 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)

Anti-MAP3K13 antibody (ab88482) 使用論文

ab88482 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab88482.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"