Anti-MAP3K12 抗体 (ab37996)


  • 製品名Anti-MAP3K12 antibody
    MAP3K12 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MAP3K12
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human MAP3K12 aa 828-859 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q12852

  • ポジティブ・コントロール
    • WB: HL-60 and SH-SY5Y cell lysates and mouse kidney tissue lysate. IHC-P: Human breast carcinoma tissue.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野


Our Abpromise guarantee covers the use of ab37996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 95 kDa.
IHC-P 1/50 - 1/100.


  • 機能May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.
  • 組織特異性Highly expressed in brain and kidney.
  • 配列類似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • ドメインInteracts with MBIP through the leucine-zipper motif.
  • 翻訳後修飾Autophosphorylated on Ser/Thr. Phosphorylated in cytosol under basal conditions and dephosphorylated when membrane-associated.
  • 細胞内局在Cytoplasm. Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dlk antibody
    • Dual leucine zipper bearing kinase antibody
    • Dual leucine zipper kinase DLK antibody
    • Leucine-zipper protein kinase antibody
    • M3K12_HUMAN antibody
    • Map3k12 antibody
    • MAPK-upstream kinase antibody
    • MEKK12 antibody
    • Mitogen-activated protein kinase kinase kinase 12 antibody
    • Mixed lineage kinase antibody
    • MUK antibody
    • Protein kinase MUK antibody
    • ZPK antibody
    • ZPKP1 antibody
    see all

Anti-MAP3K12 antibody 画像

  • All lanes : Anti-MAP3K12 antibody (ab37996) at 1/1000 dilution

    Lane 1 : HL-60 cell lysate
    Lane 2 : SH-SY5Y cell lysate

    Lysates/proteins at 35 µg per lane.

    HRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution
  • All lanes : Anti-MAP3K12 antibody (ab37996)

    Lane 1 : HL-60 cell lysate
    Lane 2 : Mouse kidney tissue lysate

    HRP-conjugated anti-rabbit IgG

    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 18 kDa,23 kDa,30 kDa,44 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MAP3K12 with ab37996. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by AEC staining.

Anti-MAP3K12 antibody (ab37996) 使用論文

ab37996 has not yet been referenced specifically in any publications.

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Application Western blot
Sample Mouse Tissue lysate - whole (Spinal Cord)
Loading amount 20 µg
Specification Spinal Cord
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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投稿 Jan 11 2012