Anti-MAP2 (phospho S136) 抗体 [EPR2361] (ab96378)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2361] to MAP2 (phospho S136)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MAP2 (phospho S136) antibody [EPR2361]
MAP2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2361] to MAP2 (phospho S136) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human fetal brain and mouse brain tissue lysates IHC-P: Human brain tissue ICC/IF: Mouse primary neuron + Alkaline Phosphatase cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2361 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96378の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 200 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF | (1) |
1/500.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 200 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/500. |
ターゲット情報
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機能
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. -
配列類似性
Contains 3 Tau/MAP repeats. -
翻訳後修飾
Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. -
細胞内局在
Cytoplasm, cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 4133 Human
- Entrez Gene: 17756 Mouse
- Entrez Gene: 25595 Rat
- Omim: 157130 Human
- SwissProt: P11137 Human
- SwissProt: P20357 Mouse
- SwissProt: P15146 Rat
- Unigene: 368281 Human
see all -
別名
- DKFZp686I2148 antibody
- MAP 2 antibody
- MAP dendrite specific antibody
see all
画像
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All lanes : Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378) at 1/1000 dilution
Lane 1 : Untreated fetal brain lysates
Lane 2 : Fetal brain lysates treated with Alkaline Phosphatase
Lane 3 : Mouse brain lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP/AP polymerized antibody
Predicted band size: 200 kDa -
Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron + Alkaline Phosphatase cells labeling MAP2 with purified ab96378 at 1/500 (3 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. -
ab96378, at a 1/100 dilution, staining MAP2 in paraffin embedded Human brain tissue by Immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab96378 は 3 報の論文で使用されています。
- Wang W et al. Toxic amyloid-ß oligomers induced self-replication in astrocytes triggering neuronal injury. EBioMedicine 42:174-187 (2019). PubMed: 30926423
- Alvarez-Carbonell D et al. Cross-talk between microglia and neurons regulates HIV latency. PLoS Pathog 15:e1008249 (2019). PubMed: 31887215
- Ni N et al. Effects of let-7b and TLX on the proliferation and differentiation of retinal progenitor cells in vitro. Sci Rep 4:6671 (2014). ICC ; Mouse . PubMed: 25327364