製品の概要

  • 製品名
    Anti-MAP2 antibody [HM-2]
    MAP2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HM-2] to MAP2
  • 由来種
    Mouse
  • アプリケーション
    適用あり: ICC, IHC-Fr, ICC/IF, IHC-FrFl, WB, IHC-P, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Cow, Human, Quail
  • 免疫原

    Other Immunogen Type corresponding to MAP2. HM-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rat brain microtubule associated proteins (MAPs).

  • ポジティブ・コントロール
    • Rat brain extract.
  • 特記事項

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab32454 as a replacement.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/500. Fix cells in 4% paraformaldehyde/PBS for 45 min; then permeabilise cells with 0.2% Triton X-100 in PBS for 5 min (see Farah et al) OR fix in 4% paraformaldehyde (containing 0.2% picric acid in 0.1 M phosphate buffer, pH 6.9) for 15 min at room temperature (see O' Hare et al) .
IHC-FrFl Use at an assay dependent concentration. PubMed: 24223856
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 280 kDa. (see Farah et al).
IHC-P Use at an assay dependent concentration.
IHC-FoFr 1/500. PubMed: 20424326

ターゲット情報

画像

  • ab11267 staining MAP2 in human hippocampal progenitor cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 5% serum for 1 hour at 18°C. Samples were incubated with the undiluted primary antibody for 12 hours at 4°C. An undiluted Alexa Fluor® 555-conjugated donkey anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunocytochemical analysis of B35 cells labelling MAP2 with ab11267 at a concentration of 2 μg/mL. The secondary was developed using Goat anti-mouse IgG. Cells were counterstained with DAPI to stain nuclei.

  • ab11267 staining rat brain tissue sections by IHC-Fr.  Sections were PFA fixed and permeabilized in TritonX100 prior to blocking with 3% BSA for 1 hour.  The rpimary antibody was diluted 1/500 and incubated with the sample for 12 hours at 4°C.  An Alexa Fluor® conjugated goat anti-mouse was used as the secondary antibody. 

    The image shows a cross section through the rat hipocampal CA1 area at magnification 200x . The anti-MAP2 staining is clearly visible in dendrites of pyramidal cells.

    See Abreview

参考文献

This product has been referenced in:
  • Paik EJ  et al. Using intracellular markers to identify a novel set of surface markers for live cell purification from a heterogeneous hIPSC culture. Sci Rep 8:804 (2018). Read more (PubMed: 29339826) »
  • Riquelme D  et al. Subcellular Localization and Activity of TRPM4 in Medial Prefrontal Cortex Layer 2/3. Front Cell Neurosci 12:12 (2018). Read more (PubMed: 29440991) »

See all 60 Publications for this product

レビューと Q&A

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunocytochemistry
Sample
Rat Cell (Cortical neurons from E17 embryos)
Permeabilization
Yes - 0.2% Triton X 5 mins
Specification
Cortical neurons from E17 embryos
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

投稿 Jun 12 2015

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Neuroblastoma (Neuro2A))
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
400000 cells
Treatment
10% FBS and fibroblast growth factor
Specification
Mouse Neuroblastoma (Neuro2A)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
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投稿 Apr 27 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Sample
Human Cell (hippocampal progenitor cells)
Specification
hippocampal progenitor cells
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Username

Dr. Aleksandra Maruszak

Verified customer

投稿 Mar 31 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Neural stem cells and progenitors)
Specification
Neural stem cells and progenitors
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton-X-100 for 30 min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Adult C57BL/6 mouse coronal brain sections)
Specification
Adult C57BL/6 mouse coronal brain sections
Username

Mr. Xiao-Dong Wang

Verified customer

投稿 Aug 03 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - TritonX100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Username

Dr. Grazyna Niewiadomska

Verified customer

投稿 Nov 23 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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