製品の概要

  • 製品名
    Anti-MAP2 antibody [AP-20]
    MAP2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AP-20] to MAP2
  • アプリケーション
    適用あり: ICC/IF, IHC-Fr, IHC-P, WB, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Xenopus laevis, Zebrafish, Quail, Salamander
  • 免疫原

    Full length native protein (purified) corresponding to Cow MAP2.

  • ポジティブ・コントロール
    • ICC: Mouse Cell (Cortical neuron culture)
  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016- GR143561. Lot numbers higher than GR143561 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/5000.
IHC-P 1/5000.

Use at an assay dependent concentration.

WB Use at an assay dependent concentration. Detects a band of approximately 280 kDa.
ICC 1/500.

ターゲット情報

  • 機能
    The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
  • 配列類似性
    Contains 3 Tau/MAP repeats.
  • 翻訳後修飾
    Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.
  • 細胞内局在
    Cytoplasm, cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp686I2148 antibody
    • MAP 2 antibody
    • MAP dendrite specific antibody
    • MAP-2 antibody
    • MAP2 antibody
    • MAP2A antibody
    • MAP2B antibody
    • MAP2C antibody
    • Microtubule associated protein 2 antibody
    • Microtubule-associated protein 2 antibody
    • MTAP2_HUMAN antibody
    see all

画像

  • Mouse monoclonal [AP-20] to MAP2 - Neuronal Marker (ab11268) used at a dilution of 1/500 in Immunocytochemistry/Immunofluorescence on Mouse Cell (Cortical neuron culture). Fixative: Paraformaldehyde. Blocking step: 2% Serum incubated for 1 hour at RT. Secondary Antibody: Goat anti-mouse AlexaFluor 594 Conjugation: Alexa Fluor® 594; 1/1000
  • ab11268 staining MAP2 in E16 mouse brain neocortex (bottom is dorsal) by immunohistochemistry. Free-floating sections were incubated with ab11268 (1/5000) for 2 hours at 21ºC. Bound antibody was detected using a biotinylated donkey anti-mouse IgG antibody. This image is courtesy of an Abreview submitted by Randal Moldrich, 6 March 2008.
  • ab11268 staining MAP2 in mouse brain tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocking with a solution containing 2% Block Ace, 0.05% Triton X-100 and PBS was performed at 250C for 1 hour. The sample was incubated with primary antibody (1/5000) in a dilution buffer containing 2% Block Ace, 0.05% Triton X-100 and PBS at 40C for 12 hours. An FITC-conjugated donkey polyclonal to mouse IgG was used as secondary antibody at 1/500 dilution.

    See Abreview

参考文献

This product has been referenced in:
  • Vicidomini C  et al. Pharmacological enhancement of mGlu5 receptors rescues behavioral deficits in SHANK3 knock-out mice. Mol Psychiatry 22:689-702 (2017). ICC/IF ; Mouse . Read more (PubMed: 27021819) »
  • Connell JW  et al. Quantitative Gait Analysis Using a Motorized Treadmill System Sensitively Detects Motor Abnormalities in Mice Expressing ATPase Defective Spastin. PLoS One 11:e0152413 (2016). Mouse . Read more (PubMed: 27019090) »

See all 12 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Sep 28 2012

Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Dr. Ruxandra Sirbulescu

Verified customer

投稿 Nov 14 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Blockace/0.05% Triton X-100/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 25 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Zebrafish Cultured Cells (Primary neuron)
Specification
Primary neuron
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X-100 10 minutes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jan 29 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (E16 Mouse brain, Free-floating sections)
Specification
E16 Mouse brain, Free-floating sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Dr. Randal Moldrich

Verified customer

投稿 Apr 22 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Cortical neuron culture)
Specification
Cortical neuron culture
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.02% Tx100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Dr. Randal Moldrich

Verified customer

投稿 Feb 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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