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Recombinant full length protein corresponding to Cow MAP2. Recombinant bovine MAP2 protein expressed in and purified from E. Coli
Our Abpromise guarantee covers the use of ab92434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/20000. Predicted molecular weight: 280 kDa.|
|ICC||Use at an assay dependent concentration.|
ab92434 staining MAP2 in mouse brain neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/2500 in PBS) for 24 hours at 4°C. ab150169, a goat anti-chicken Alexa Fluor® 488 (1/500) was used as the secondary antibody.