製品の概要

  • 製品名
  • 製品の詳細
    Chicken polyclonal to MAP2
  • アプリケーション
    適用あり: WB, ICC/IF, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    Recombinant full length protein corresponding to Cow MAP2. Recombinant bovine MAP2 protein expressed in and purified from E. Coli

  • ポジティブ・コントロール
    • Rat cortex lysate; mixed neuron/glial cultures.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/20000. Predicted molecular weight: 280 kDa.
ICC/IF 1/2500.
ICC Use at an assay dependent concentration.

ターゲット情報

画像

  • ab92434 staining MAP2 in mouse brain neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/2500 in PBS) for 24 hours at 4°C. ab150169, a goat anti-chicken Alexa Fluor® 488 (1/500) was used as the secondary antibody.

    See Abreview

  • Anti-MAP2 antibody (ab92434) at 1/20000 dilution + Rat cerebral cortex lysate

    Predicted band size : 280 kDa
  • ab92434 at 1/2500 dilution staining MAP2 in mixed neuron/glial cultures. by Immunofluorescence. The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody (red). Cell nuclei are visualized with DAPI DNA stain.

参考文献

This product has been referenced in:
  • Sellgren CM  et al. Patient-specific models of microglia-mediated engulfment of synapses and neural progenitors. Mol Psychiatry 22:170-177 (2017). ICC/IF ; Human . Read more (PubMed: 27956744) »
  • Westbroek W  et al. A new glucocerebrosidase-deficient neuronal cell model provides a tool to probe pathophysiology and therapeutics for Gaucher disease. Dis Model Mech 9:769-78 (2016). ICC . Read more (PubMed: 27482815) »

See all 7 Publications for this product

レビューと Q&A

Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Sample
Mouse Cell (Brain, neurons)
Specification
Brain, neurons
Permeabilization
No
Fixative
Paraformaldehyde
Username

Ms. Kirsty Ireland

Verified customer

投稿 Mar 19 2014

Thank you for contacting us.

For this chicken polyclonal MAP2 antibody the concentration is roughly between 15 and 30µg per 100µl, however, we have not determined the absolute concentration. This is because some antibodies can have high conc...

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Vielen Dank für Ihre Antwort und für diese weiteren Informationen. Es tut mir wirklich leid zu hören, dass die MAP2- und Cy5-Antikörper in der Immunfluoreszenz mit PFA-Fixierung nicht funktionieren. Beide Antikörper haben unsere strikten Qualitätsk...

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Vielen Dank für Ihren Anruf. Es tut mir leid zu hören, dass Sie Probleme mit unserem MAP2-Antikörper ab92434 haben. Wie besprochen habe ich unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Fragebogens erhalten ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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