製品の概要

  • 製品名
  • 製品の詳細
    Chicken polyclonal to MAP2
  • アプリケーション
    適用あり: ELISA, IHC-Fr, IHC-FoFr, IHC-P, WB, ICC/IF, IHC (PFA fixed)more details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Cow, Dog, Human, Cynomolgus monkey, Common marmoset, Aplysia
  • 免疫原

    Full length native protein (purified) corresponding to Cow MAP2.

  • ポジティブ・コントロール
    • ICC: cultured rat cortical neurons

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/5000.
IHC-Fr Use at an assay dependent dilution.
IHC-FoFr 1/500. PubMed: 18684835
IHC-P Use at an assay dependent concentration.
WB 1/100000. This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands.
ICC/IF 1/10000.
IHC (PFA fixed) Use at an assay dependent concentration.

ターゲット情報

画像

  • PFA-fixed, paraffin embedded sections of sheep cerebellum were stained for MAP2 with ab5392 at 1/2000 dilution in immunohistochemical analysis. Goat Anti-Chicken IgY H&L (Biotin) (ab6876) was used as secondary antibody at 1/200 dilution.

     

  • Rat E20 cultured cortical neuron-glial cells stained for MAP2 (red) using ab5392 at 1/2000 dilution for ICC/IF. Tau is detected with a mouse monoclonal anti-Tau antibody (green). The nuclear counter stain is DAPI (blue). Overlap of MAP2 and Tau staining results in an orange-yellow color.

  • ab5392 staining MAP2 in murine brain tissue sections by Immunohistochemistry (PFA fixed).
    Boiling in citrate-buffer was used as antigen retrieval method. ab5392 used at a 1/2000 dilution for 12 hours. The secondary used was an Alexa-Fluor 555 conjugated goat anti-chicken polyclonal used at a 1/400 dilution.

    See Abreview

  • All lanes : Anti-MAP2 antibody (ab5392) at 1/50000 dilution

    Lane 1 : Adult rat brain lysate
    Lane 2 : Embryonic E20 rat brain lysate
    Lane 3 : Adult mouse brain lysate
  • ab5392 detecting MAP2 recombinant protein by direct ELISA. Mouse recombinat protien was coated on to microplate in carbonate coating buffer pH 9.6 for 1 hour at 37°C, Plate were blocked with 3% BSA for 1 hour at 37°C and incubated with the primary antibody (1/5000 in PBS + 1% Tween-20) for 1 hour at 37°C. An alkaline phosphatase Goat anti-chicken IgG polyclonal (1/100000) was used as the secondary antibody.

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  • PFA-fixed, paraffin embedded sections of rat brain were stained for MAP2 with ab5392 at 1/4000 dilution in immunohistochemical analysis. Goat Anti-Chicken IgY H&L (Biotin) (ab6876) was used as secondary antibody at 1/200 dilution.

     

  • ab5392 at a dilution of 1/10000, staining MAP 2 (green) in tissue cultured rat cortical neurons by immunofluorescence. Nuclei are stained blue with Dapi.
  • Rat cortical neurons and glia in mixed tissue culture stained with ab5392 (Chicken antibody to MAP2) (green)(1:30 000), a mouse monoclonal antibody to GFAP (red) and nuclei of all cells stained with Hoechst dye (blue).
  • ab5392 staining MAP2 in rat spinal cord tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde and permeablized with 50% Ethanol for 30 minutes. The sample was incubated with primary antibody (1/250 in PBS + 0.3% Triton X-100) at 4°C for 48 hours. An Alexa Fluor® 488-conjugated goat anti-chicken IgY polyclonal (1/1000) was used as the secondary antibody.

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  • PFA-fixed, paraffin embedded sections of dog brain were stained for MAP2 with ab5392 at 1/2000 dilution in immunohistochemical analysis. Goat Anti-Chicken IgY H&L (Biotin) (ab6876) was used as secondary antibody at 1/200 dilution.

  • ab5392 at 1/5000 staining PFA fixed free floating sections of mouse brain.  An AlexaFluor® 633 conjugated goat anti-chicken antibody was used as the secondary.

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  • ab5392 staining MAP2 in mouse neurons by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.4% Triton X-100 prior to blocking in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/10000 and incubated with the sample for 20 hours at 21°C. Alexa fluor® 488 goat polyclonal to chicken IgY, diluted 1/400, was used as the secondary antibody.

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  • Anti-MAP2 antibody (ab5392) at 1/10000 dilution + Mouse brain lysate - post nuclear supernatant at 10 µg

    Secondary
    HRP-conjugated rabbit anti-chicken IgY at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 70,280 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

参考文献

This product has been referenced in:
  • Kreiner G  et al. Lack of riluzole efficacy in the progression of the neurodegenerative phenotype in a new conditional mouse model of striatal degeneration. PeerJ 5:e3240 (2017). IHC-P ; Mouse . Read more (PubMed: 28462043) »
  • Yuan J  et al. M2 microglia promotes neurogenesis and oligodendrogenesis from neural stem/progenitor cells via the PPAR? signaling pathway. Oncotarget 8:19855-19865 (2017). ICC/IF ; Mouse . Read more (PubMed: 28423639) »

See all 172 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step
None
Sample
Rat Tissue sections (Spinal cord)
Specification
Spinal cord
Permeabilization
Yes - 50% Ethanol 30 min
Fixative
Paraformaldehyde
Username

Mr. Tom Owen

Verified customer

投稿 Apr 08 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA PH9.0
Permeabilization
Yes - 0.1% TritonX-100
Specification
Brain
Blocking step
Cas-Block(Invitrogen) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 25 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Temporal Cortex)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
No
Specification
Temporal Cortex
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Kathleen .

Verified customer

投稿 Feb 22 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (Olfactory stem cells)
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory stem cells
Blocking step
BSA 3% + Goat serum 5% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 18 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (Olfactory stem cells)
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory stem cells
Blocking step
BSA 3% + Goat serum 5% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Antoine Veron

Verified customer

投稿 Nov 18 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Olfactory stem cells GFP)
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory stem cells GFP
Blocking step
BSA 3% + Goat serum 5% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 18 2016

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (NSCs (Neural Stem cells))
Permeabilization
Yes - tritonx100
Specification
NSCs (Neural Stem cells)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Kyungjoo Seong

Verified customer

投稿 Nov 02 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Neuron)
Permeabilization
Yes - TX100 0.05%
Specification
Neuron
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 20 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Ferret Tissue sections (P0-ferret-parietal cortex)
Permeabilization
Yes - 0.5% Triton in 1x PBS for 1 hour5% Triton in 1x PBS for 1 hour
Specification
P0-ferret-parietal cortex
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Francis Djankpa

Verified customer

投稿 Apr 29 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Ferret Tissue sections (parietal cortex in Brain coronal slices)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1x Citrate Buffer
Permeabilization
Yes - 0.5% Triton in 1x PBS for 1 hour
Specification
parietal cortex in Brain coronal slices
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Francis Djankpa

Verified customer

投稿 Mar 03 2016

1-10 of 47 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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