製品の概要

  • 製品名Anti-MAP1LC3A antibody [EP1528Y]
    MAP1LC3A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1528Y] to MAP1LC3A
  • アプリケーション適用あり: WB, IP, ICC, IHC-Pmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues on the N-terminus of human MAPILC3A was used as an immogen.

  • ポジティブ・コントロール
    • Fetal brain cytoplasmic cell lysate Human brain tissue
  • 特記事項

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab52628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50000. Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa).
IP 1/100.
ICC 1/50 - 1/100.
IHC-P Use at an assay dependent concentration.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機��Probably involved in formation of autophagosomal vacuoles (autophagosomes).
    • 組織特異性Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
    • 配列類似性Belongs to the MAP1 LC3 family.
    • 翻訳後修飾The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
    • 細胞内局在Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ATG8E antibody
      • Autophagy-related protein LC3 A antibody
      • Autophagy-related ubiquitin-like modifier LC3 A antibody
      • LC3 antibody
      • LC3A antibody
      • MAP1 light chain 3 like protein 1 antibody
      • MAP1 light chain 3-like protein 1 antibody
      • MAP1A/1B light chain 3 A antibody
      • MAP1A/MAP1B LC3 A antibody
      • MAP1A/MAP1B light chain 3 A antibody
      • MAP1ALC3 antibody
      • MAP1BLC3 antibody
      • Map1lc3a antibody
      • Microtubule associated proteins 1A/1B light chain 3 antibody
      • Microtubule-associated protein 1 light chain 3 alpha antibody
      • Microtubule-associated proteins 1A and 1B, light chain 3 antibody
      • Microtubule-associated proteins 1A/1B light chain 3A antibody
      • MLP3A_HUMAN antibody
      see all

    Anti-MAP1LC3A antibody [EP1528Y] 画像

    • Anti-MAP1LC3A antibody [EP1528Y] (ab52628) at 1/50000 dilution + Fetal brain lysate at 10 µg

      Secondary
      goat anti-rabbit HRP labelled at 1/2000 dilution

      Predicted band size : 14 kDa
      Observed band size : 16 kDa (why is the actual band size different from the predicted?)
    • ab52628, at a 1/50 dilution, staining Human MAPILC3A in brain using Immunohistochemistry, Paraffin Embedded Tissue.
    • IHC image of ab52628 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52628, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-MAP1LC3A antibody [EP1528Y] (ab52628) 使用論文

    This product has been referenced in:
    • Koukourakis MI  et al. Autophagosome Proteins LC3A, LC3B and LC3C Have Distinct Subcellular Distribution Kinetics and Expression in Cancer Cell Lines. PLoS One 10:e0137675 (2015). ICC/IF ; Human . Read more (PubMed: 26378792) »
    • Kim JY  et al. Expression of autophagy-related proteins according to androgen receptor and HER-2 status in estrogen receptor-negative breast cancer. PLoS One 9:e105666 (2014). IHC-P ; Human . Read more (PubMed: 25140630) »

    See all 3 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (nerublastoma)
    Specification nerublastoma
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 16°C
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    投稿 Apr 28 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Tissue lysate - whole (Liver)
    Loading amount 30 µg
    Specification Liver
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    投稿 Jun 25 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Liver)
    Loading amount 30 µg
    Specification Liver
    Gel Running Conditions Reduced Denaturing (12)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    投稿 Jun 25 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Tissue lysate - whole (Lung)
    Loading amount 50 µg
    Specification Lung
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    投稿 Apr 02 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (kidney)
    Loading amount 30 µg
    Specification kidney
    Gel Running Conditions Reduced Denaturing (12)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    投稿 Jun 13 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Cell lysate - whole cell (liver)
    Loading amount 50 µg
    Specification liver
    Gel Running Conditions Reduced Denaturing (12)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    投稿 Jun 08 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Tissue lysate - whole (liver)
    Loading amount 15 µg
    Specification liver
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    投稿 Jan 05 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Tissue lysate - whole (liver, heart, brain, lung, muscle, small bowel, sp)
    Loading amount 20 µg
    Specification liver, heart, brain, lung, muscle, small bowel, sp
    Gel Running Conditions Non-reduced Denaturing (15)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    投稿 Jun 05 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"