製品の概要

  • 製品名
    Anti-MAP1B antibody [AA6]
    MAP1B 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AA6] to MAP1B
  • 特異性
    Monoclonal Anti-MAP1b does not react with tubulin of other Microtubule associated proteins.
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Hamster, Cow, Cat, Human
  • 免疫原

    Rat brain microtubule associated proteins.

  • ポジティブ・コントロール
    • Rat brain.
  • 特記事項
    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC/IF
  • 追加情報
    ICC/IF: Use at an assay dependent dilution in conjunction with either immunofluorescence or immunoperoxidase labelling methods.
    WB: 1/500. Predicted molecular weight: 270 kDa. May run higher due to many phosphorylation sites: 320kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Facilitates tyrosination of alpha-tubulin in neuronal microtubules (By similarity). Phosphorylated MAP1B may play a role in the cytoskeletal changes that accompany neurite extension. Possibly MAP1B binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules. Acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation and membrane blebbing.
    • 配列類似性
      Belongs to the MAP1 family.
    • ドメイン
      Has a highly basic region with many copies of the sequence KKEE and KKEI/V, repeated but not at fixed intervals, which is responsible for the binding of MAP1B to microtubules.
    • 翻訳後修飾
      LC1 is generated from MAP1B by proteolytic processing.
      S-nitrosylation at Cys-2464 enhances interaction with microtubules, and may act as an effector modification for neuronal nitric oxide synthase control of growth-cone size, growth-cone collapse and axon retraction.
    • 細胞内局在
      Cytoplasm, cytoskeleton. Cytoplasm. Cell junction, synapse. Cell projection, dendritic spine. Colocalizes with DAPK1 in the microtubules and cortical actin fibers.
    • Information by UniProt
    • 参照データベース
    • 別名
      • FUTSCH antibody
      • LC1 antibody
      • MAP-1B antibody
      • MAP1 light chain LC1 antibody
      • Map1b antibody
      • MAP1B_HUMAN antibody
      • MAP5 antibody
      • Microtubule associated protein 1B antibody
      • Mtap1b antibody
      • Mtap5 antibody
      see all

    画像

    • ab11266 (1/300) staining MAP1B (centre column) in Human cortical neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde. Left column staining: tubulin, actin, synapsin, SAP102. Right column: overlay.

    参考文献

    This product has been referenced in:
    • Eriksson M  et al. MAP1B binds to the NMDA receptor subunit NR3A and affects NR3A protein concentrations. Neurosci Lett 475:33-7 (2010). WB, ICC/IF ; Rat . Read more (PubMed: 20304030) »
    • Impens F  et al. Mechanistic insight into taxol-induced cell death. Oncogene 27:4580-91 (2008). WB ; Human . Read more (PubMed: 18408750) »

    See all 6 Publications for this product

    レビューと Q&A

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    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.01M Citrate buffer pH6.0+Tween 0.05%
    Sample
    Mouse Tissue sections (brain)
    Specification
    brain
    Permeabilization
    Yes - 0.2%Triton X100
    Fixative
    Paraformaldehyde
    Username

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    投稿 Aug 08 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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