Mouse, Rat, Chicken, Hamster, Cow, Cat, Human
Rat brain microtubule associated proteins.
Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution in conjunction with either immunofluorescence or immunoperoxidase labelling methods.
WB: 1/500. Predicted molecular weight: 270 kDa. May run higher due to many phosphorylation sites: 320kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Facilitates tyrosination of alpha-tubulin in neuronal microtubules (By similarity). Phosphorylated MAP1B may play a role in the cytoskeletal changes that accompany neurite extension. Possibly MAP1B binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules. Acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation and membrane blebbing.
Belongs to the MAP1 family.
Has a highly basic region with many copies of the sequence KKEE and KKEI/V, repeated but not at fixed intervals, which is responsible for the binding of MAP1B to microtubules.
LC1 is generated from MAP1B by proteolytic processing. S-nitrosylation at Cys-2464 enhances interaction with microtubules, and may act as an effector modification for neuronal nitric oxide synthase control of growth-cone size, growth-cone collapse and axon retraction.
Cytoplasm, cytoskeleton. Cytoplasm. Cell junction, synapse. Cell projection, dendritic spine. Colocalizes with DAPK1 in the microtubules and cortical actin fibers.
Immunocytochemistry/ Immunofluorescence - Anti-MAP1B antibody [AA6] (ab11266)Image from Eriksson M et al., Neurosci Lett, 475(1), 33-7. Fig 2.; doi: 10.1016/j.neulet.2010.03.039. with permission from Elsevier.
ab11266 (1/300) staining MAP1B (centre column) in Human cortical neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde. Left column staining: tubulin, actin, synapsin, SAP102. Right column: overlay.