製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MAP1A
  • アプリケーション
    適用あり: IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1400 - 1500 of Human MAP1A.

    (Peptide available as ab107891.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in human hippocampus formalin fixed paraffin embedded tissue section (Immunohistochemistry)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Structural protein involved in the filamentous cross-bridging between microtubules and other skeletal elements.
  • 組織特異性
    Brain.
  • 配列類似性
    Belongs to the MAP1 family.
  • ドメイン
    The basic region containing the repeats may be responsible for the binding of MAP1A to microtubules.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    LC2 is generated from MAP1A by proteolytic processing.
  • 細胞内局在
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MAP 1A antibody
    • MAP 1L antibody
    • MAP-1A antibody
    • MAP1 light chain LC2 antibody
    • Map1a antibody
    • MAP1A_HUMAN antibody
    • MAP1L antibody
    • Microtubule associated protein 1 like antibody
    • Microtubule associated protein 1A antibody
    • MTAP 1A antibody
    • MTAP1A antibody
    • Proliferation related protein p80 antibody
    • Proliferation-related protein p80 antibody
    see all

画像

  • IHC image of ab89648 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89648, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ICC/IF image of ab89648 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab89648 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

ab89648 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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