製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Map17
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Pig, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Mouse Map17.

    (Peptide available as ab37556.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the Recombinant Human Map17 protein.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab31405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 33 kDa corresponding to the molecular weight of recombinant Map17 (predicted molecular weight: 12 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    画像

    • Anti-Map17 antibody (ab31405) at 1 µg/ml + Map17 Human Recombinant Protein at 0.1 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 12 kDa
      Observed band size: 33 kDa (why is the actual band size different from the predicted?)



      ab31405 gave a positive signal against the partial recombinant Map17 protein with GST tag which has an expected molecular weight of 33 kDa.

    プロトコール

    参考文献

    This product has been referenced in:
    • Shao Y  et al. EGFR-TKI resistance and MAP17 are associated with cancer stem cell like properties. Oncol Lett 15:6655-6665 (2018). Read more (PubMed: 29616128) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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