Anti-Mannosidase II 抗体 (ab77353)

製品の概要

  • 製品名Anti-Mannosidase II antibody
    Mannosidase II 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Mannosidase II
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    KISSDIKSQNR

    , corresponding to internal sequence amino acids 881-891 of Human Mannosidase II

  • ポジティブ・コントロール
    • Human Placenta lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 131 kDa).
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
  • パスウェイProtein modification; protein glycosylation.
  • 配列類似性Belongs to the glycosyl hydrolase 38 family.
  • 細胞内局在Golgi apparatus membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 3-1 antibody
    • 6-alpha-mannosidase antibody
    • Alpha mannosidase 2 antibody
    • alpha Mannosidase II antibody
    • Alpha-mannosidase 2 antibody
    • AMAN II antibody
    • Golgi alpha mannosidase II antibody
    • Golgi alpha-mannosidase II antibody
    • Golgi integral membrane protein 7 antibody
    • GOLIM7 antibody
    • MA2A1_HUMAN antibody
    • MAN II antibody
    • Man2a1 antibody
    • MANA 2 antibody
    • MANA2 antibody
    • MANII antibody
    • Mann II antibody
    • Mannosidase alpha class 2A member 1 antibody
    • Mannosidase Two antibody
    • Mannosidase, alpha type II antibody
    • Mannosidase, alpha, II antibody
    • Mannosyl oligosaccharide 1 3 1 6 alpha mannosidase antibody
    • Mannosyl oligosaccharide 1,3 1,6 alpha mannosidase antibody
    • Mannosyl-oligosaccharide 1 antibody
    see all

Anti-Mannosidase II antibody 画像

  • Anti-Mannosidase II antibody (ab77353) at 0.3 µg/ml + Human Placenta lysate (in RIPA buffer) at 35 µg

    Predicted band size : 131 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Mannosidase II antibody (ab77353) at 3 µg/ml

    Lane 1 : MOCK-transfected HEK293 cell lysate
    Lane 2 : HEK293 cell lysate overexpressing Mannosidase II


    Predicted band size : 131 kDa

Anti-Mannosidase II antibody (ab77353) 使用論文

ab77353 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human dermal fibroblasts)
Loading amount 10 µg
Specification human dermal fibroblasts
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Aug 04 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"