Rabbit polyclonal to Mannosidase II
Chimpanzee, Gorilla, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 750 - 850 of Human Mannosidase II.
This antibody gave a positive signal in the following tissue lysates: Human Skeletal Muscle; Human Liver; Human Placenta.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).
Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
Protein modification; protein glycosylation.
Belongs to the glycosyl hydrolase 38 family.
Golgi apparatus membrane.
Information by UniProt
Alpha mannosidase 2 antibody
Western blot - Anti-Mannosidase II antibody (ab113448)
All lanes :
Anti-Mannosidase II antibody (ab113448) at 1 µg/ml
Lane 1 :
Human skeletal muscle tissue lysate - total protein (
Lane 2 :
Human liver tissue lysate - total protein (
Lane 3 :
Human placenta tissue lysate - total protein (
Lysates/proteins at 10 µg per lane.
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
Additional bands at :
52 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Exposure time :
has not yet been referenced specifically in any publications.
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