リコンビナント
RabMAb

Anti-Mannose Receptor 抗体 [EPR6828(B)] (ab125028)

製品の概要

  • 製品名
    Anti-Mannose Receptor antibody [EPR6828(B)]
    Mannose Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6828(B)] to Mannose Receptor
  • アプリケーション
    適用あり: WB, Flow Cyt, ICC/IFmore details
    適用なし: IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide within Human Mannose Receptor aa 1400 to the C-terminus. The exact sequence is proprietary.
    Database link: P22897

  • ポジティブ・コントロール
    • WB: BxPC3, Caco2, HepG2 and 293T lysates; Human fetal liver tissue lysate. ICC/IF: MOLT-4 cells
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • 解離定数(KD 値)
    KD = 1.75 x 10 -10 M
    Learn more about KD
  • バッファー
    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR6828(B)
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab125028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 166 kDa).
Flow Cyt Use at an assay dependent concentration.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 1 µg/ml.
  • 追加情報
    Is unsuitable for IHC-P or IP.
  • ターゲット情報

    • 機能
      Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
    • 配列類似性
      Contains 8 C-type lectin domains.
      Contains 1 fibronectin type-II domain.
      Contains 1 ricin B-type lectin domain.
    • 細胞内局在
      Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • bA541I19.1 antibody
      • C type lectin domain family 13 member D antibody
      • C-type lectin domain family 13 member D antibody
      • CD 206 antibody
      • CD206 antibody
      • CD206 antigen antibody
      • CLEC13D antibody
      • CLEC13DL antibody
      • Macrophage mannose receptor 1 antibody
      • Macrophage mannose receptor 1 like protein 1 antibody
      • Macrophage mannose receptor antibody
      • Mannose receptor C type 1 antibody
      • Mannose receptor C type 1 like 1 antibody
      • MMR antibody
      • MRC 1 antibody
      • MRC1 antibody
      • MRC1_HUMAN antibody
      • MRC1L1 antibody
      • OTTHUMP00000045206 antibody
      see all

    画像

    • Immunocytochemistry/Immunofluorescence analysis MOLT-4 (Human lymphoblastic leukemia) cells labeling Mannose Receptor with ab125028 at 1 μg/mL. Cells were fixed with 100% methanol. ab150081, an AlexaFluor®488 Goat anti-Rabbit at 1/1000 was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/250) - Microtubule Marker (Alexa Fluor® 594). Nuclei were counterstained with DAPI (blue).

      Confocal image showing membranous staining on MOLT-4 cell line.

    • All lanes : Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028) at 1/1000 dilution

      Lane 1 : BxPC3 lysate
      Lane 2 : Caco2 lysate
      Lane 3 : HepG2 lysate
      Lane 4 : Human fetal liver lysate
      Lane 5 : 293T lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 166 kDa
    • Flow Cytometry analysis of Molt-4 (human acute lymphoblastic leukemia ) cells labeling Mannose Receptor with unpurified ab125028 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    参考文献

    This product has been referenced in:
    • Subramanian S  et al. TREM-1 associated macrophage polarization plays a significant role in inducing insulin resistance in obese population. J Transl Med 15:85 (2017). IHC-P, IF ; Human . Read more (PubMed: 28454543) »
    • Crawford JR  et al. Plasma Levels of Endothelial Microparticles Bearing Monomeric C-reactive Protein are Increased in Peripheral Artery Disease. J Cardiovasc Transl Res 9:184-93 (2016). IF ; Human . Read more (PubMed: 26891844) »

    See all 3 Publications for this product

    レビューと Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human Macrophages Type 2)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    20 µg
    Specification
    Human Macrophages Type 2
    Blocking step
    Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
    Username

    Dr. Francesco Elia Marino

    Verified customer

    投稿 Mar 21 2016

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (muscle)
    Specification
    muscle
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Formaldehyde
    Username

    Mr. Thomas Zwakhals

    Verified customer

    投稿 Mar 15 2016

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Permeabilization
    Yes - 0.1 M PBS with 3% Triton X
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    投稿 Jun 25 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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