製品の概要

  • 製品名
    Anti-Mannose Receptor antibody [15-2]
    Mannose Receptor 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [15-2] to Mannose Receptor
  • アプリケーション
    適用あり: Flow Cyt, IHC-Fr, WB, IHC-R, IHC-FoFr, Inhibition Assay, ICC/IFmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse, Horse, Human
  • 免疫原

    Purified human mannose receptor from human placental tissue.

  • エピトープ
    Stains extracellular domain of Mannose Receptor.
  • ポジティブ・コントロール
    • Macrophages IHC: liver endothelial cells and Kupffer cells, spermatids, spermatozoa
  • 特記事項

    The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 特記事項(精製)
    0.2 µm filtered antibody solution
  • 一次抗体 備考
    The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells.
  • ポリ/モノ
    モノクローナル
  • クローン名
    15-2
  • ミエローマ
    unknown
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/50. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/50.
WB 1/50. Detects a band of approximately 175 kDa (predicted molecular weight: 166 kDa). This antibody can be use for ligand-receptor intervention studies.
IHC-R Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration. Antibody 15-2 functions as an inhibitor of binding. The antibody was functionally tested by preincubation of immobilized receptor with antibody. This prevented binding of t-PA to immobilized receptor.
ICC/IF Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能
      Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
    • 配列類似性
      Contains 8 C-type lectin domains.
      Contains 1 fibronectin type-II domain.
      Contains 1 ricin B-type lectin domain.
    • 細胞内局在
      Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • bA541I19.1 antibody
      • C type lectin domain family 13 member D antibody
      • C-type lectin domain family 13 member D antibody
      • CD 206 antibody
      • CD206 antibody
      • CD206 antigen antibody
      • CLEC13D antibody
      • CLEC13DL antibody
      • Macrophage mannose receptor 1 antibody
      • Macrophage mannose receptor 1 like protein 1 antibody
      • Macrophage mannose receptor antibody
      • Mannose receptor C type 1 antibody
      • Mannose receptor C type 1 like 1 antibody
      • MMR antibody
      • MRC 1 antibody
      • MRC1 antibody
      • MRC1_HUMAN antibody
      • MRC1L1 antibody
      • OTTHUMP00000045206 antibody
      see all

    画像

    • Frozen sections of human tonsil. ab8918 was used in a dilution of 1/25 and shows staining of endothelia of lymph vessels.

    • ab8918 staining Mannose Receptor in human mammary gland tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with 70% Ethanol and blocking for 10 minutes at RT was performed. The sample was incubated with primary antibody (1/10) for 1 hour. A HRP-conjugated mouse polyclonal to mouse IgG was used undiluted as secondary antibody.

      See Abreview

    • ab8918 staining the Mannose Receptor in Human nasal polyp and tonsil tissue sections by Immunohistochemistry (resin-embedded sections). The tissue was fixed in acetone and inhibitors and embedded into GMA resin. Samples were incubated with primary antibody (1/400 in Tris buffered saline) for 18 hours at 21°C. A Biotin-conjugated Rabbit anti-mouse polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • Immunohistochemical analysis of murine uterus tissue sections, staining Mannose Receptor with ab8918.

      Tissue was fixed with paraformaldehyde and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/40 in 1% BSA) for 1.5 hours. An undiluted HRP-conjugated rabbit anti-mouse polyclonal IgG was used as the secondary antibody.

      See Abreview

    • ab8918 staining Mannose Receptor in human endometriosis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/10 in PBS + 1% NGS) for 16 hours at 4°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

      See Abreview

    参考文献

    This product has been referenced in:
    • Dragano NRV  et al. Polyunsaturated fatty acid receptors, GPR40 and GPR120, are expressed in the hypothalamus and control energy homeostasis and inflammation. J Neuroinflammation 14:91 (2017). Read more (PubMed: 28446241) »
    • Guo X  et al. Hypoxia promotes glioma-associated macrophage infiltration via periostin and subsequent M2 polarization by upregulating TGF-beta and M-CSFR. Oncotarget 7:80521-80542 (2016). IHC, ICC/IF ; Human . Read more (PubMed: 27602954) »

    See all 22 Publications for this product

    レビューと Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Sample
    Human Tissue sections (inflamed human colon)
    Specification
    inflamed human colon
    Permeabilization
    No
    Fixative
    Acetone
    Username

    Abcam user community

    Verified customer

    投稿 Dec 01 2014

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Sample
    Human Tissue sections (Endometriosis)
    Specification
    Endometriosis
    Permeabilization
    No
    Fixative
    Acetone
    Username

    Mrs. Aimée-Lys Rusesabagina

    Verified customer

    投稿 Sep 12 2014

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Sample
    Mouse Tissue sections (Breast tumor)
    Specification
    Breast tumor
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Dr. Gajendra Katara

    Verified customer

    投稿 Sep 05 2014

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Sample
    Mouse Cell (Peritoneal macrophages)
    Specification
    Peritoneal macrophages
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Aug 20 2014

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3 · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Sample
    Mouse Tissue sections (Uterus)
    Specification
    Uterus
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Dr. Mukesh Jaiswal

    Verified customer

    投稿 Nov 12 2013

    Thank you for contacting us.

    Both of these product are tested and guaranteed to work in Flow Cytometry. These are specific against extracellular domain so the permeabilization step is optional; the antibodies would be able to stain the cells w...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Uterus)
    Specification
    Uterus
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Username

    Dr. Mukesh Jaiswal

    Verified customer

    投稿 Sep 21 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (placenta)
    Specification
    placenta
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 40 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Dr. Varkha Agrawal

    Verified customer

    投稿 Sep 18 2012

    Thank you very much for your interest in our antibodies.

    To our knowledge, ab8918 and ab64693have not been tested in ELISA and also not in sandwichELISA.

    The epitope of ab8918 was not mapped and the same is true for ab117644.
    ...

    Read More

    Thank you for contacting us. I am very glad to hear that you will be using our products in your experiments. I can gladly recommend three biotin labeled antibodies to suit your needs and have placed links for biotin labeled antibodies below. I would...

    Read More

    1-10 of 22 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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