製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MAN1A2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within middle amino acids 468-517 (FALGADGSRA DKAGHYLELG AEIARTCHES YDRTALKLGP ESFKFDGAVE) of human MAN1A2 (NP_006690).

  • ポジティブ・コントロール
    • Human fetal brain lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性
    MAN1A2 plays a role in the maturation of Asn-linked oligosaccharides (N-glycans) in mammalian cells, by progressively excising alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2).
  • 細胞内局在
    Golgi apparatus membrane; Single-pass type II membrane protein
  • 参照データベース
  • 別名
    • Alpha 1,2 mannosidase IB antibody
    • MAN1B antibody
    • Mannosidase alpha class 1A member 2 antibody
    • Mannosyl oligosaccharide 1,2 alpha mannosidase IB antibody
    • Processing alpha 1,2 mannosidase IB antibody
    see all

画像

  • Anti-MAN1A2 antibody (ab84590) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 73 kDa
    Observed band size : 73 kDa
    Additional bands at : 40 kDa,60 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab84590 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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