Anti-MAN1 抗体 (ab121854)
Key features and details
- Rabbit polyclonal to MAN1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MAN1 antibody -
製品の詳細
Rabbit polyclonal to MAN1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
antigen sequence, corresponding to C terminal amino acids 766-902 of Human MAN1.
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ポジティブ・コントロール
- IHC-P: Human kidney tissue; WB: Daudi and HeLa cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121854の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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特記事項 |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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機能
Can function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. Antagonizes TGF-beta-induced cell proliferation arrest. -
組織特異性
Heart, brain, placenta, lung, liver and skeletal muscle. -
関連疾患
Defects in LEMD3 are the cause of Buschke-Ollendorff syndrome (BOS) [MIM:166700]; also known as dermatoosteopoikilosis or disseminated dermatofibrosis with osteopoikilosis or dermatofibrosis lenticularis disseminata with osteopoikilosis or osteopathia condensans disseminata. BOS refers to the association of osteopoikilosis with disseminated connective-tissue nevi. Osteopoikilosis is a skeletal dysplasia characterized by a symmetric but unequal distribution of multiple hyperostotic areas in different parts of the skeleton. Both elastic-type nevi (juvenile elastoma) and collagen-type nevi (dermatofibrosis lenticularis disseminata) have been described in BOS. Skin or bony lesions can be absent in some family members, whereas other relatives may have both.
Defects in LEMD3 are a cause of melorheostosis (MEL) [MIM:155950]. Melorheostosis is a rare mesenchymal dysplasia and one of the sclerosing bone disorders. It is caused by a developmental error, with a sclerotomal distribution, frequently involving one limb. It may be asymptomatic, but pain, stiffness with limitation of motion, leg-length discrepancy and limb deformity may occur. -
配列類似性
Contains 1 LEM domain. -
細胞内局在
Nucleus inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 23592 Human
- Omim: 607844 Human
- SwissProt: Q9Y2U8 Human
- Unigene: 728281 Human
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別名
- Inner nuclear membrane protein Man1 antibody
- LEM domain containing protein 3 antibody
- LEM domain-containing protein 3 antibody
see all
画像
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ab121854, at a 1/50 dilution, staining MAN1 in human rectum shows strong nuclear and cytoplasmic positivity in glandular cells.
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All lanes : Anti-MAN1 antibody (ab121854) at 0.4 µg/ml
Lane 1 : Daudi (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab121854 は 1 報の論文で使用されています。
- Nastaly P et al. Role of the nuclear membrane protein Emerin in front-rear polarity of the nucleus. Nat Commun 11:2122 (2020). PubMed: 32358486