Mammalian Cell Extraction Kit (ab65399)

製品の概要

  • 製品名Mammalian Cell Extraction Kit
  • テスト
    500 x 1 assay
  • サンプルの種類
    Tissue, Adherent cells, Suspension cells
  • アッセイタイプDirect
  • 全工程の試験時間
    0h 20m
  • 製品の概要

    Abcam's Mammalian Cell Extraction Kit provides optimized cell extraction buffer, protease inhibitor cocktail, and DTT for convenient extraction of mammalian proteins from cultured cells and tissue samples, under nondenaturing conditions. Cell lysate prepared using the kit can be used in a variety of applications, such as enzyme activity assays (e.g. caspase activity assays), Western blot analysis, and others. The entire procedure takes less than 20 minutes.
    Visit our FAQs page for tips and troubleshooting.

製品の特性

  • 関連性Mammalian Cell Extraction methods allow extraction of mammalian proteins from cultured cells and tissue samples.

プロトコール

Mammalian Cell Extraction Kit (ab65399) 使用論文

ab65399 has not yet been referenced specifically in any publications.

Product Wall

For tissue samples: Start with 10-100 mg of the tissue, add 500-1000 μl (or ~4-6 volumes) of the assay buffer on ice, homogenize using a Dounce homogenizer (10-50 passes) on ice, until efficient lysis is confirmed, by viewing the cells under the mic...

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Mammalian Cell Extraction Kit

Below average Excellent 5/5 (Ease of Use)
Abreviews
This is a good kit for a rapid protein extraction. However, it is important to know that it is not performing better than a general RIPA buffer or EGFR buffer. Additionally, the protease inhibitor provided with the kit does not contain phosphatase inhibitor. all this info need to be taken in consideration for the selection of a good buffer.

Graph shows comparison between Abcam and RIPA buffer. RIPA buffer performed better in both COV434 and LnCAP with no difference when samples were sonicated using a bath sonicator or probe sonicator.
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Dr. FRANCESCO ELIA MARINO

Verified customer

投稿 Aug 27 2014

I double checked with the lab to answer this question.

1. I am happy to confirm that this kit can be used to isolate both membrane and cytoplasmatic proteins. The membrane proteins are in the pellet from step 5. If you would like to also isol...

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Thank you for your inquiry.

Please find the protocol for this kit below:

Mammalian Protein Extraction Protocol

A. General Consideration and Reagent Preparation:

- After opening the kit, store Cell Extraction Buff...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"